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Literature summary for 2.5.1.47 extracted from

  • Bonner, E.R.; Cahoon, R.E.; Knapke, S.M.; Jez, J.M.
    Molecular basis of cysteine biosynthesis in plants: Structural and functional analysis of O-acetylserine sulfhydrylase from Arabidopsis thaliana (2005), J. Biol. Chem., 280, 38803-38813.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
native protein and mutant K46A with pyridoxal 5’-phosphate and methionine covalently linked as an external aldimine Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
H157N comparable to wild-type Arabidopsis thaliana
H157Q reduced kcat-value, reduced Km-value Arabidopsis thaliana
K46A no enzymic activity, crystallization data Arabidopsis thaliana
N77A reduced kcat-value Arabidopsis thaliana
N77D drastically reduced kcat-value Arabidopsis thaliana
Q147A drastically reduced kcat-value, increased Km-value Arabidopsis thaliana
Q147E drastically reduced kcat-value, increased Km-value Arabidopsis thaliana
S269A reduced kcat-value, reduced Km-value Arabidopsis thaliana
S269T reduced kcat-value, reduced Km-value Arabidopsis thaliana
S75A drastically reduced kcat-value Arabidopsis thaliana
S75N drastically reduced kcat-value Arabidopsis thaliana
S75T drastically reduced kcat-value Arabidopsis thaliana
T182A reduced kcat-value Arabidopsis thaliana
T182S comparable to wild-type Arabidopsis thaliana
T185A drastically reduced kcat-value Arabidopsis thaliana
T185S drastically reduced kcat-value Arabidopsis thaliana
T74A drastically reduced kcat-value Arabidopsis thaliana
T74S reduced kcat-value Arabidopsis thaliana
T78A reduced kcat-value, reduced Km-value Arabidopsis thaliana
T78S reduced kcat-value, reduced Km-value Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
Sulfide mutant N77D, pH 7.0, 25°C Arabidopsis thaliana
0.21
-
Sulfide mutant S75T, pH 7.0, 25°C Arabidopsis thaliana
0.22
-
Sulfide wild-type, pH 7.0, 25°C Arabidopsis thaliana
0.22
-
Sulfide mutant T182S, pH 7.0, 25°C Arabidopsis thaliana
0.22
-
Sulfide mutant T185A, pH 7.0, 25°C Arabidopsis thaliana
0.23
-
Sulfide mutant N77A, pH 7.0, 25°C Arabidopsis thaliana
0.27
-
Sulfide mutant Q147S, pH 7.0, 25°C Arabidopsis thaliana
0.3
-
Sulfide mutant H157Q, pH 7.0, 25°C Arabidopsis thaliana
0.3
-
Sulfide mutant T78S, pH 7.0, 25°C Arabidopsis thaliana
0.36
-
Sulfide mutant T185S, pH 7.0, 25°C Arabidopsis thaliana
0.39
-
Sulfide mutant T182A, pH 7.0, 25°C Arabidopsis thaliana
0.4
-
Sulfide mutant H157N, pH 7.0, 25°C Arabidopsis thaliana
0.44
-
Sulfide mutant T78A, pH 7.0, 25°C Arabidopsis thaliana
0.54
-
Sulfide mutant S269A, pH 7.0, 25°C Arabidopsis thaliana
0.57
-
O3-acetyl-L-serine mutant S269A, pH 7.0, 25°C Arabidopsis thaliana
0.68
-
O3-acetyl-L-serine mutant T78A, pH 7.0, 25°C Arabidopsis thaliana
0.82
-
O3-acetyl-L-serine mutant T78S, pH 7.0, 25°C Arabidopsis thaliana
0.91
-
O3-acetyl-L-serine mutant H157Q, pH 7.0, 25°C Arabidopsis thaliana
0.94
-
O3-acetyl-L-serine mutant H157N, pH 7.0, 25°C Arabidopsis thaliana
0.96
-
O3-acetyl-L-serine mutant S269T, pH 7.0, 25°C Arabidopsis thaliana
1.2
-
O3-acetyl-L-serine mutant S75T, pH 7.0, 25°C Arabidopsis thaliana
1.2
-
O3-acetyl-L-serine mutant T182A, pH 7.0, 25°C Arabidopsis thaliana
1.4
-
O3-acetyl-L-serine wild-type, pH 7.0, 25°C Arabidopsis thaliana
1.5
-
O3-acetyl-L-serine mutant T185A, pH 7.0, 25°C Arabidopsis thaliana
1.5
-
O3-acetyl-L-serine mutant T74S, pH 7.0, 25°C Arabidopsis thaliana
1.6
-
Sulfide mutant S269T, pH 7.0, 25°C Arabidopsis thaliana
1.6
-
O3-acetyl-L-serine mutant S75A, pH 7.0, 25°C Arabidopsis thaliana
1.6
-
O3-acetyl-L-serine mutant T182S, pH 7.0, 25°C Arabidopsis thaliana
1.6
-
O3-acetyl-L-serine mutant T185S, pH 7.0, 25°C Arabidopsis thaliana
1.6
-
O3-acetyl-L-serine mutant T74A, pH 7.0, 25°C Arabidopsis thaliana
1.7
-
Sulfide mutant Q147A, pH 7.0, 25°C Arabidopsis thaliana
2
-
O3-acetyl-L-serine mutant S75N, pH 7.0, 25°C Arabidopsis thaliana
3.2
-
Sulfide mutant T74S, pH 7.0, 25°C Arabidopsis thaliana
3.8
-
O3-acetyl-L-serine mutant Q147S, pH 7.0, 25°C Arabidopsis thaliana
3.9
-
Sulfide mutant T74A, pH 7.0, 25°C Arabidopsis thaliana
4.4
-
O3-acetyl-L-serine mutant Q147A, pH 7.0, 25°C Arabidopsis thaliana
4.6
-
Sulfide mutant S75A, pH 7.0, 25°C Arabidopsis thaliana
4.7
-
O3-acetyl-L-serine mutant N77A, pH 7.0, 25°C Arabidopsis thaliana
5.7
-
Sulfide mutant S75N, pH 7.0, 25°C Arabidopsis thaliana
10.2
-
O3-acetyl-L-serine mutant N77D, pH 7.0, 25°C Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
O3-acetyl-L-serine + hydrogensulfide
-
Arabidopsis thaliana L-cysteine + acetate
-
?

Subunits

Subunits Comment Organism
dimer crystallization data Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.08
-
O3-acetyl-L-serine mutant T74A, pH 7.0, 25°C Arabidopsis thaliana
0.09
-
O3-acetyl-L-serine mutant S75N, pH 7.0, 25°C Arabidopsis thaliana
0.19
-
O3-acetyl-L-serine mutant Q147S, pH 7.0, 25°C Arabidopsis thaliana
0.22
-
Sulfide mutant Q147S, pH 7.0, 25°C Arabidopsis thaliana
0.34
-
O3-acetyl-L-serine mutant N77D, pH 7.0, 25°C Arabidopsis thaliana
0.42
-
Sulfide mutant T74A, pH 7.0, 25°C Arabidopsis thaliana
0.73
-
Sulfide mutant N77D, pH 7.0, 25°C Arabidopsis thaliana
1.07
-
O3-acetyl-L-serine mutant T185A, pH 7.0, 25°C Arabidopsis thaliana
1.44
-
Sulfide mutant T185A, pH 7.0, 25°C Arabidopsis thaliana
1.45
-
Sulfide mutant S75N, pH 7.0, 25°C Arabidopsis thaliana
3.11
-
O3-acetyl-L-serine mutant T185S, pH 7.0, 25°C Arabidopsis thaliana
4.02
-
O3-acetyl-L-serine mutant Q147A, pH 7.0, 25°C Arabidopsis thaliana
5.57
-
Sulfide mutant T185S, pH 7.0, 25°C Arabidopsis thaliana
7.16
-
O3-acetyl-L-serine mutant S75A, pH 7.0, 25°C Arabidopsis thaliana
12.5
-
Sulfide mutant Q147A, pH 7.0, 25°C Arabidopsis thaliana
20.2
-
Sulfide mutant S75A, pH 7.0, 25°C Arabidopsis thaliana
29.5
-
Sulfide mutant S269A, pH 7.0, 25°C Arabidopsis thaliana
34.3
-
O3-acetyl-L-serine mutant S269A, pH 7.0, 25°C Arabidopsis thaliana
51.5
-
O3-acetyl-L-serine mutant S75T, pH 7.0, 25°C Arabidopsis thaliana
82.7
-
Sulfide mutant S75T, pH 7.0, 25°C Arabidopsis thaliana
155
-
Sulfide mutant N77A, pH 7.0, 25°C Arabidopsis thaliana
370
-
Sulfide mutant T78S, pH 7.0, 25°C Arabidopsis thaliana
403
-
O3-acetyl-L-serine mutant N77A, pH 7.0, 25°C Arabidopsis thaliana
456
-
O3-acetyl-L-serine mutant T74S, pH 7.0, 25°C Arabidopsis thaliana
537
-
Sulfide mutant T78A, pH 7.0, 25°C Arabidopsis thaliana
554
-
O3-acetyl-L-serine mutant T78A, pH 7.0, 25°C Arabidopsis thaliana
572
-
O3-acetyl-L-serine mutant S269T, pH 7.0, 25°C Arabidopsis thaliana
775
-
O3-acetyl-L-serine mutant T78S, pH 7.0, 25°C Arabidopsis thaliana
889
-
O3-acetyl-L-serine mutant T182A, pH 7.0, 25°C Arabidopsis thaliana
929
-
Sulfide mutant S269T, pH 7.0, 25°C Arabidopsis thaliana
1055
-
O3-acetyl-L-serine mutant H157Q, pH 7.0, 25°C Arabidopsis thaliana
1520
-
O3-acetyl-L-serine mutant H157N, pH 7.0, 25°C Arabidopsis thaliana
1600
-
Sulfide mutant T182A, pH 7.0, 25°C Arabidopsis thaliana
1660
-
O3-acetyl-L-serine mutant T182S, pH 7.0, 25°C Arabidopsis thaliana
1690
-
Sulfide mutant T182S, pH 7.0, 25°C Arabidopsis thaliana
1700
-
Sulfide mutant T74S, pH 7.0, 25°C Arabidopsis thaliana
1780
-
O3-acetyl-L-serine wild-type, pH 7.0, 25°C Arabidopsis thaliana
1780
-
Sulfide mutant H157Q, pH 7.0, 25°C Arabidopsis thaliana
1990
-
Sulfide mutant H157N, pH 7.0, 25°C Arabidopsis thaliana
2170
-
Sulfide wild-type, pH 7.0, 25°C Arabidopsis thaliana