Cloned (Comment) | Organism |
---|---|
expression of isoenzyme A, B and C in Escherichia coli | Arabidopsis sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0526 | - |
cysteine | pH 7.5, 37°C, isoenzyme C | Arabidopsis sp. | |
0.0986 | - |
cysteine | isoenzyme A | Arabidopsis sp. | |
0.113 | - |
cysteine | pH 7.5, 37°C, isoenzyme B | Arabidopsis sp. | |
0.7 | - |
O-acetyl-L-Ser | pH 7.5, 37°C, isoenzyme B | Arabidopsis sp. | |
2.1 | - |
O-acetyl-L-Ser | pH 7.5, 37°C, isoenzyme A | Arabidopsis sp. | |
3.9 | - |
O-acetyl-L-Ser | pH 7.5, 37°C, isoenzyme C | Arabidopsis sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | isoenzyme A | Arabidopsis sp. | 5737 | - |
mitochondrion | isoenzyme C | Arabidopsis sp. | 5739 | - |
plastid | isoenzyme B | Arabidopsis sp. | 9536 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cysteine + dithiothreitol | Arabidopsis sp. | the side reaction of the enzyme seems to contribute massively to the total H2S release of higher plants at least at higher pH values | S-(2,3-hydroxy-4-thiobutyl)-L-cysteine + H2S | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Arabidopsis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cysteine + dithiothreitol | - |
Arabidopsis sp. | S-(2,3-hydroxy-4-thiobutyl)-L-cysteine + H2S | - |
? | |
L-cysteine + dithiothreitol | the side reaction of the enzyme seems to contribute massively to the total H2S release of higher plants at least at higher pH values | Arabidopsis sp. | S-(2,3-hydroxy-4-thiobutyl)-L-cysteine + H2S | - |
? | |
O-acetyl-L-Ser + H2S | - |
Arabidopsis sp. | L-Cys + acetate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
recombinant isoenzyme C | Arabidopsis sp. |
8 | - |
recombinant isoenzyme A and B | Arabidopsis sp. |