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Literature summary for 2.5.1.47 extracted from
- Purification and characterization of O-acetylserine (thiol) lyase from spinach chloroplasts (1992), Arch. Biochem. Biophys., 295, 379-390.
General Stability
| General Stability | Organism |
|---|---|
| chloroplast enzyme very unstable | Spinacia oleracea |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.25 | - |
H2S | pH 7.5, 25°C, chloroplast enzyme | Spinacia oleracea |  |
| 1.3 | - |
O-acetyl-L-Ser | pH 7.5, 25°C, chloroplast enzyme | Spinacia oleracea | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | different from cytosolic and mitochondrial enzymes, as revealed by polyclonal antibody studies, 3-5% of total activity | Spinacia oleracea | 9507 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
2 * 35000, SDS-PAGE | Spinacia oleracea |
| 68000 | - |
chloroplast enzyme, gel filtration | Spinacia oleracea |
| 310000 | - |
cysteine synthetase complex, gel filtration | Spinacia oleracea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Spinacia oleracea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 96 | - |
chloroplast enzyme | Spinacia oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| O-acetyl-L-serine | - |
Spinacia oleracea | L-cysteine + acetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 35000, SDS-PAGE | Spinacia oleracea |
| More | amino acid composition | Spinacia oleracea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
chloroplast enzyme | Spinacia oleracea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Spinacia oleracea |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | 1.1 mol per mol subunit | Spinacia oleracea | |
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Release 2023.1 (January 2023)
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