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Literature summary for 2.5.1.47 extracted from
- Purification and physicochemical characterization of O-acetyl-L-serine sulfhydrylase from Chlamydomonas reinhardtii (1953), Plant Sci., 53, 93-99.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| very unstable | Chlamydomonas reinhardtii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| hydroxylamine | - |
Chlamydomonas reinhardtii |  |
| L-cysteine | - |
Chlamydomonas reinhardtii | |
| methionine | - |
Chlamydomonas reinhardtii | |
| p-hydroxymercuribenzoate | - |
Chlamydomonas reinhardtii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.75 | - |
H2S | pH 7.5, 50°C | Chlamydomonas reinhardtii | |
| 2 | - |
O-acetyl-L-serine | pH 7.5, 50°C | Chlamydomonas reinhardtii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
sedimentation equilibrium | Chlamydomonas reinhardtii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chlamydomonas reinhardtii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Chlamydomonas reinhardtii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 41.15 | - |
- |
Chlamydomonas reinhardtii |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20 C, phosphate buffer, pH 7.5, glycerol, pyridoxal-5'-phosphate, 8 days, 50% loss of activity | Chlamydomonas reinhardtii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| O-acetyl-L-serine | - |
Chlamydomonas reinhardtii | L-cysteine + acetate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Chlamydomonas reinhardtii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Chlamydomonas reinhardtii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | protects against inactivation | Chlamydomonas reinhardtii | |
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Release 2023.1 (January 2023)
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