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Literature summary for 2.5.1.47 extracted from

  • Rosichan, J.L.; Blake, N.; Stallard, R.; Owais, W.M.; Kleinhofs, A.; Nilan, R.A.
    O-acetylserine (thiol)-lyase from barley converts sodium azide to a mutagenic metabolite (1983), Biochim. Biophys. Acta, 748, 367-373.
No PubMed abstract available
Search for more literature for 2.5.1.47

Inhibitors

Inhibitors Comment Organism Structure
NaN3
-
 Hordeum vulgare
O-acetylserine at 150 mM Hordeum vulgare

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3
-
 NaN3 pH 7.6, 25°C Hordeum vulgare
8.2
-
 O-acetyl-L-Ser pH 7.6, 25°C Hordeum vulgare

Organism

Organism UniProt Comment Textmining
Hordeum vulgare
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Hordeum vulgare

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5500
-
-
 Hordeum vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NaN3 + O-acetyl-Ser
-
 Hordeum vulgare beta-azidoalanine + sodium acetate mutagenic in Salmonella typhimurium ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 10 min, no loss of activity Hordeum vulgare
60
-
 10 min, 85% loss of activity Hordeum vulgare

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Hordeum vulgare

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3
-
 NaN3 pH 7.4, 25 C Hordeum vulgare