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Literature summary for 2.5.1.47 extracted from

  • Hock Ng, B.; Anderson, J.W.
    Chloroplast cysteine synthases of Trifolium repens and Pisum sativum (1978), Biochemistry, 17, 879-885.
No PubMed abstract available
Search for more literature for 2.5.1.47

Inhibitors

Inhibitors Comment Organism Structure
cystathionine
-
 Pisum sativum
cystathionine 91% inhibition at 10 mM Trifolium repens
L-cysteine
-
 Pisum sativum
L-cysteine 66% inhibition at 10 mM Trifolium repens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information various methods compared Pisum sativum
additional information
-
 additional information various methods compared Trifolium repens
0.24
-
 H2S pH 7.8, 35° C Pisum sativum
0.51
-
 H2S pH 7.8, 35°C Trifolium repens
3.1
-
 O-acetyl-L-Ser pH 7.8, 35°C Pisum sativum
3.5
-
 O-acetyl-L-serine pH 7.8, 35°C Trifolium repens

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast 68-86% of activity, stroma Pisum sativum 9507
-
chloroplast 68-86% of activity, stroma Trifolium repens 9507
-

Organism

Organism UniProt Comment Textmining
Pisum sativum
-
-
-
Trifolium repens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pisum sativum
-
 Trifolium repens

Renatured (Commentary)

Renatured (Comment) Organism
-
 Pisum sativum
-
 Trifolium repens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.91
-
-
 Trifolium repens

Storage Stability

Storage Stability Organism
-15 C, potassium phosphate buffer, dithiothreitol, one week, 50% loss of activity Trifolium repens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
O-acetyl-L-serine
-
 Pisum sativum L-cysteine + acetate
-
 ?
O-acetyl-L-serine
-
 Trifolium repens L-cysteine + acetate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 potassium phosphate buffer Pisum sativum
7.8
-
 potassium phosphate buffer Trifolium repens

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate restores activity after dialysis against cysteine Pisum sativum
pyridoxal 5'-phosphate restores activity after dialysis against cysteine Trifolium repens