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Literature summary for 2.5.1.47 extracted from

  • Kredich, N.M.; Becker, M.A.
    Cysteine biosynthesis: serine transacetylase and O-acetylserine sulfhydrylase (1971), Methods Enzymol., 17 B, 459-470.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
H2S less than Salmonella enterica subsp. enterica serovar Typhimurium
0.12
-
acetyl-CoA
-
Salmonella enterica subsp. enterica serovar Typhimurium
0.12
-
acetyl-CoA
-
Escherichia coli
0.7
-
L-Ser
-
Salmonella enterica subsp. enterica serovar Typhimurium
0.7
-
L-Ser
-
Escherichia coli
5
-
O-acetyl-L-Ser free enzyme Salmonella enterica subsp. enterica serovar Typhimurium
20
-
O-acetyl-L-Ser complex-bound enzyme Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68000
-
free enzyme, sedimentation equilibrium Salmonella enterica subsp. enterica serovar Typhimurium
309000
-
cysteine synthetase complex, sedimentation equilibrium Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
O-acetyl-L-Ser + sulfide Salmonella enterica subsp. enterica serovar Typhimurium final step in Cys synthesis L-Cys + acetate
-
?
O-acetyl-L-Ser + sulfide Escherichia coli final step in Cys synthesis L-Cys + acetate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
one enzyme free, one associated with serine transacetylase in cysteine synthase
-

Purification (Commentary)

Purification (Comment) Organism
-
Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
292
-
complex-bound enzyme Salmonella enterica subsp. enterica serovar Typhimurium
1100
-
free enzyme Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

Storage Stability Organism
-20 C, Tris-HCl buffer, pH 7.6, bovine serum albumine, 8 months, 10% loss of activity Salmonella enterica subsp. enterica serovar Typhimurium
20 C, room temperature, Tris-HCl buffer, pH 7.6, bovine serum albumine, several days, no loss of activity Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Cys + acetate
-
Salmonella enterica subsp. enterica serovar Typhimurium O-acetyl-L-Ser + H2S
-
r
L-Cys + acetate
-
Escherichia coli O-acetyl-L-Ser + H2S
-
r
O-acetyl-L-Ser + H2S
-
Salmonella enterica subsp. enterica serovar Typhimurium L-Cys + acetate
-
r
O-acetyl-L-Ser + H2S
-
Escherichia coli L-Cys + acetate
-
r
O-acetyl-L-Ser + methyl mercaptan
-
Salmonella enterica subsp. enterica serovar Typhimurium S-methylcysteine + acetate product identification uncertain ir
O-acetyl-L-Ser + sulfide final step in Cys synthesis Salmonella enterica subsp. enterica serovar Typhimurium L-Cys + acetate
-
?
O-acetyl-L-Ser + sulfide final step in Cys synthesis Escherichia coli L-Cys + acetate
-
?
O-acetyl-L-serine
-
Salmonella enterica subsp. enterica serovar Typhimurium L-cysteine + acetate
-
?

Subunits

Subunits Comment Organism
dimer
-
Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
62
-
10 min, 5% loss of activity Salmonella enterica subsp. enterica serovar Typhimurium
67
-
10 min, 50% loss of activity Salmonella enterica subsp. enterica serovar Typhimurium
70
-
10 min, 70% loss of activity Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
733
-
O-acetyl-L-Ser
-
Salmonella enterica subsp. enterica serovar Typhimurium
733
-
O-acetyl-L-Ser
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate 1 per subunit of free enzyme, 4 per of cysteine synthase complex Salmonella enterica subsp. enterica serovar Typhimurium