Literature summary for 2.5.1.46 extracted from

Belda-Palazon, B.; Nohales, M.A.; Rambla, J.L.; Acena, J.L.; Delgado, O.; Fustero, S.; Martinez, M.C.; Granell, A.; Carbonell, J.; Ferrando, A.
Biochemical quantitation of the eIF5A hypusination in Arabidopsis thaliana uncovers ABA-dependent regulation (2014), Front. Plant Sci., 5, 202.

Search for more literature for 2.5.1.46

Cloned(Commentary)

Cloned (Comment)	Organism
gene dhs, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3), coexpression with His-tagged DOHH (deoxyhypusine hydroxylase) in Escherichia coli strain BL21-CodonPlus (DE3), quantitative real-time PCR expression analysis	Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
44000	-	x * 44000, recombinant His-tagged enzyme, SDS-PAGE	Arabidopsis thaliana

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q9FI94	gene dhs	-
Arabidopsis thaliana Col-0	Q9FI94	gene dhs	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21-CodonPlus (DE3) by nickel affinity chromatography and ultrafiltration, followed by cleavage of the His-tag through protease 3C and elimination via affinity chromatography	Arabidopsis thaliana

Subunits

Subunits	Comment	Organism
?	x * 44000, recombinant His-tagged enzyme, SDS-PAGE	Arabidopsis thaliana

Synonyms

Synonyms	Comment	Organism
deoxyhypusinesynthase	-	Arabidopsis thaliana
DHS	-	Arabidopsis thaliana

General Information

General Information	Comment	Organism
physiological function	the plant stress hormone abscisic acid causes a reduction in the hypusination of eIF5A1 probably through the post-transcriptional alteration of DHS activity	Arabidopsis thaliana

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Release 2023.1 (January 2023)