Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.46 extracted from

  • Wolff, E.C.; Lee, S.B.; Park, M.H.
    Assay of deoxyhypusine synthase activity (2011), Methods Mol. Biol., 720, 195-205.
    View publication on PubMedView publication on EuropePMC

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[eIF5A-precursor]-lysine + spermidine Homo sapiens
-
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information development of a radioactive assay method for detection of spermidine incorporation into eIF5A, overview Homo sapiens ?
-
?
[eIF5A-precursor]-lysine + spermidine
-
Homo sapiens [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
?
[eIF5A-precursor]-lysine + spermidine DHS catalyzes a NAD-dependent cleavage between N4 and C5 of spermidine and the transfer of the butylamine moiety to the ?-amino group of a specific lysine residue of the eIF5A precursor, eIF5A(Lys), to form the deoxyhypusine residue. The enzyme is modification is strictly specific for one single lysine residue of the cellular protein eukaryotic initiation factor 5A (eIF5A) and does not work on short peptides Homo sapiens [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine 1,3-diaminopropane is released as a byproduct from spermidine cleavage ?

Synonyms

Synonyms Comment Organism
DHS
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.2
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+ required Homo sapiens

General Information

General Information Comment Organism
physiological function deoxyhypusine synthase catalyzes the synthesis of deoxyhypusine [N?-(4-aminobutyl)-lysine], the first step in the post-translational synthesis of an unusual amino acid hypusine [N?-(4-amino-2-hydroxybutyl)-lysine] Homo sapiens