Protein Variants | Comment | Organism |
---|---|---|
D238A | NAD-site mutant, less than 5% activity of wild-type | Homo sapiens |
D243A | spermidine-site mutant, no spermidine-binding | Homo sapiens |
D313A | unable to bind eIF5A-precursor, no synthesis of deoxyhypusine | Homo sapiens |
D316A | spermidine-site mutant, no spermidine-binding | Homo sapiens |
D342A | NAD-site mutant, less than 5% activity of wild-type | Homo sapiens |
E137A | NAD-site mutant, less than 5% activity of wild-type | Homo sapiens |
E323A | spermidine-site mutant, no spermidine-binding | Homo sapiens |
G283A | NAD-site mutant, activity similar to wild-type | Homo sapiens |
H288A | spermidine-site mutant, no spermidine-binding | Homo sapiens |
K305A | spermidine-site mutant with retained avtivity | Homo sapiens |
K329A | spermidine-site mutant, 18-24% spermidine binding capacity | Homo sapiens |
S317A | NAD-site mutant, activity similar to wild-type | Homo sapiens |
T308A | NAD-site mutant, activity similar to wild-type | Homo sapiens |
W327A | spermidine-site mutant, no spermidine-binding | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[eIF5A-precursor]-lysine + spermidine | - |
Homo sapiens | [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine | i.e. [eIF5A-precursor]-N6-(4-aminobutyl)lysine | ? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | one enzyme tetramer can bind up to four each of NAD+ and spermidine, the binding of spermidine being dependent on NAD+ | Homo sapiens | |
NADH | - |
Homo sapiens |