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Literature summary for 2.5.1.44 extracted from

  • Tholl, D.; Ober, D.; Martin, W.; Kellermann, J.; Hartmann, T.
    Purification, molecular cloning and expression in Escherichia coli of homospermidine synthase from Rhodopseudomonas viridis (1996), Eur. J. Biochem., 240, 373-379.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli BL21, which originally does not possess HSS activity. 40-50% of the soluble protein in crude extracts are detected as homospermidine synthase Blastochloris viridis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
NAD+
-
Blastochloris viridis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
2 * 52000, SDS-PAGE Blastochloris viridis
52600
-
2 * 52600, calculated from the amino acid sequence Blastochloris viridis
100000
-
gel filtration Blastochloris viridis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 putrescine Blastochloris viridis
-
sym-homospermidine + NH3
-
?

Organism

Organism UniProt Comment Textmining
Blastochloris viridis
-
expressed in Escherichia coli strain BL21/pHsRvT72.2
-
Blastochloris viridis
-
DSM 134
-
Blastochloris viridis
-
accession no. L77975
-

Purification (Commentary)

Purification (Comment) Organism
224fold Blastochloris viridis
overexpressed Escherichia coli strain BL21 Blastochloris viridis

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
Blastochloris viridis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.97
-
-
Blastochloris viridis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 putrescine
-
Blastochloris viridis sym-homospermidine + NH3
-
?

Subunits

Subunits Comment Organism
dimer 2 * 52000, SDS-PAGE Blastochloris viridis
dimer 2 * 52600, calculated from the amino acid sequence Blastochloris viridis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Blastochloris viridis