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Literature summary for 2.5.1.44 extracted from

  • Srivenugopal, K.S.; Adiga, P.R.
    Enzymic synthesis of sym-homospermidine in Lathyrus sativus (grass pea) seedlings (1980), Biochem. J., 190, 461-464.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.44

Inhibitors

Inhibitors Comment Organism Structure
1,3-diaminopropane
-
 Lathyrus sativus
cadaverine
-
 Lathyrus sativus
N-ethylmaleimide inhibition suggests, that the enzyme is of a thiol nature Lathyrus sativus
spermidine
-
 Lathyrus sativus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3
-
 putrescine
-
 Lathyrus sativus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
 gel filtration Lathyrus sativus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 putrescine Lathyrus sativus as partially purified enzymes have been used in the assay the dependence on spermidine for the homospermidine synthesis may have been overlooked. If these enzymes should proof to be spermidine-dependent like other enzymes from plant sources, they must be classified as EC 2.5.1.45 sym-homospermidine + NH3
-
 ?
2 putrescine Santalum album as partially purified enzymes have been used in the assay the dependence on spermidine for the homospermidine synthesis may have been overlooked. If these enzymes should proof to be spermidine-dependent like other enzymes from plant sources, they must be classified as EC 2.5.1.45 sym-homospermidine + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Lathyrus sativus
-
 grass pea
-
Santalum album
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial, 100fold Lathyrus sativus

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Santalum album
-
seedling
-
 Lathyrus sativus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00025
-
-
 Lathyrus sativus
0.0038
-
-
 Santalum album

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 putrescine as partially purified enzymes have been used in the assay the dependence on spermidine for the homospermidine synthesis may have been overlooked. If these enzymes should proof to be spermidine-dependent like other enzymes from plant sources, they must be classified as EC 2.5.1.45 Lathyrus sativus sym-homospermidine + NH3
-
 ?
2 putrescine as partially purified enzymes have been used in the assay the dependence on spermidine for the homospermidine synthesis may have been overlooked. If these enzymes should proof to be spermidine-dependent like other enzymes from plant sources, they must be classified as EC 2.5.1.45 Santalum album sym-homospermidine + NH3
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 reaction linear up to 60 min and is proportional to the amount of enzyme protein (0.1-0.5 mg) Lathyrus sativus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
 stable for at least 2 weeks in the presence of 1 mM NAD+ Lathyrus sativus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4
-
 in Tris/HCl buffer Lathyrus sativus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Santalum album
NAD+ absolute requirement Lathyrus sativus