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Literature summary for 2.5.1.43 extracted from

  • Dreyfus, C.; Pignol, D.; Arnoux, P.
    Expression, purification, crystallization and preliminary X-ray analysis of an archaeal protein homologous to plant nicotianamine synthase (2008), Acta Crystallogr. Sect. F, 64, 933-935.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Methanothermobacter thermautotrophicus

Crystallization (Commentary)

Crystallization (Comment) Organism
diffraction to 1.7 A resolution, space group P212121 Methanothermobacter thermautotrophicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
and 60000, gel filtration Methanothermobacter thermautotrophicus
60000
-
and 30000, gel filtration Methanothermobacter thermautotrophicus

Organism

Organism UniProt Comment Textmining
Methanothermobacter thermautotrophicus
-
-
-
Methanothermobacter thermautotrophicus MTH675
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information enzyme dimers are linked by at least one disulfide bridge Methanothermobacter thermautotrophicus

Purification (Commentary)

Purification (Comment) Organism
recombinant, His-tagged protein Methanothermobacter thermautotrophicus

Subunits

Subunits Comment Organism
dimer and monomer, 2 * 30000, SDS-PAGE, 2 * 3304, calculated, His-tagged protein Methanothermobacter thermautotrophicus
monomer and dimer, 1 * 30000, SDS-PAGE, 1 * 3304, calculated, His-tagged protein Methanothermobacter thermautotrophicus