Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
x * 30000, SDS-PAGE | Hordeum vulgare |
40000 | 50000 | gel filtration | Hordeum vulgare |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine | Hordeum vulgare | synthesis of nicotianamide, the key precursor of the mugineic acid family phytosiderophores | ? | - |
? | |
S-adenosyl-L-methionine | Hordeum vulgare | strongly induced by Fe-deficiency treatment | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Hordeum vulgare |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | primary roots | Hordeum vulgare | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Hordeum vulgare |
Storage Stability | Organism |
---|---|
4°C, in 50 mM Tris, 1 mM EDTA, 3 mM dithiothreitol, pH 8.7 with HCl, stable for about 1 week | Hordeum vulgare |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine | - |
Hordeum vulgare | 5'-S-methyl-5'-thioadenosine + nicotianamine | - |
? | |
S-adenosyl-L-methionine | synthesis of nicotianamide, the key precursor of the mugineic acid family phytosiderophores | Hordeum vulgare | ? | - |
? | |
S-adenosyl-L-methionine | strongly induced by Fe-deficiency treatment | Hordeum vulgare | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000, SDS-PAGE | Hordeum vulgare |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Hordeum vulgare |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | - |
overnight in 50 mM Tris, 1 mM EDTA, 3 mM dithiothreitol, pH 8.7 with HCl, 90% loss of activity | Hordeum vulgare |
5.5 | - |
overnight in 50 mM Tris, 1 mM EDTA, 3 mM dithiothreitol, pH 8.7 with HCl, 80% loss of activity | Hordeum vulgare |
7 | - |
activity is almost completely recovered after overnight exposure in 50 mM Tris, 1 mM EDTA, 3 mM dithiothreitol, pH 8.7 with HCl | Hordeum vulgare |