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Literature summary for 2.5.1.43 extracted from

  • Higuchi, K.; Kanazawa, K.; Nishizawa, N.K.; Chino, M.; Mori, S.
    Purification and characterization of nicotianamine synthase from Fe-deficient barley roots (1994), Plant Soil, 165, 173-179.
No PubMed abstract available

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
x * 30000, SDS-PAGE Hordeum vulgare
40000 50000 gel filtration Hordeum vulgare

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine Hordeum vulgare synthesis of nicotianamide, the key precursor of the mugineic acid family phytosiderophores ?
-
?
S-adenosyl-L-methionine Hordeum vulgare strongly induced by Fe-deficiency treatment ?
-
?

Organism

Organism UniProt Comment Textmining
Hordeum vulgare
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Hordeum vulgare

Source Tissue

Source Tissue Comment Organism Textmining
root primary roots Hordeum vulgare
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Hordeum vulgare

Storage Stability

Storage Stability Organism
4°C, in 50 mM Tris, 1 mM EDTA, 3 mM dithiothreitol, pH 8.7 with HCl, stable for about 1 week Hordeum vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine
-
Hordeum vulgare 5'-S-methyl-5'-thioadenosine + nicotianamine
-
?
S-adenosyl-L-methionine synthesis of nicotianamide, the key precursor of the mugineic acid family phytosiderophores Hordeum vulgare ?
-
?
S-adenosyl-L-methionine strongly induced by Fe-deficiency treatment Hordeum vulgare ?
-
?

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE Hordeum vulgare

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Hordeum vulgare

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
overnight in 50 mM Tris, 1 mM EDTA, 3 mM dithiothreitol, pH 8.7 with HCl, 90% loss of activity Hordeum vulgare
5.5
-
overnight in 50 mM Tris, 1 mM EDTA, 3 mM dithiothreitol, pH 8.7 with HCl, 80% loss of activity Hordeum vulgare
7
-
activity is almost completely recovered after overnight exposure in 50 mM Tris, 1 mM EDTA, 3 mM dithiothreitol, pH 8.7 with HCl Hordeum vulgare