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Literature summary for 2.5.1.42 extracted from

  • Hemmi, H.; Shibuya, K.; Takahashi, Y.; Nakayama, T.; Nishino, T.
    (S)-2,3-Di-O-geranylgeranylglyceryl phosphate synthase from the thermoacidophilic archaeon Sulfolobus solfataricus. Molecular cloning and characterization of a membrane-intrinsic prenyltransferase involved in the biosynthesis of archaeal ether-linked membrane lipids (2004), J. Biol. Chem., 279, 50197-50203.
    View publication on PubMed
Search for more literature for 2.5.1.42

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli with a hexameric histidine tag Saccharolobus solfataricus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane integral membrane protein Saccharolobus solfataricus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ divalent metal ion required, optimal activity with 10 mM Mg2+. 40% of maximal activity is obtrained with 10 mM Ca2+. 1 mM Ca2+ hardly activates Saccharolobus solfataricus
Mg2+ requirement of a divalent metal ion for activity. 1 mM Mg2+ gives 40% of the activity with 10 mM Mg2+ Saccharolobus solfataricus
Mn2+ divalent metal ion required, optimal activity with 10 mM Mg2+. 10 mM Mn2+ leads to severely decreased activity Saccharolobus solfataricus
Zn2+ divalent metal ion required, optimal activity with 10 mM Mg2+. 10 mM Zn2+ leads to severely decreased activity. 1 mM Zn2+ hardly activates Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 x * 28000, SDS-PAGE Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranylgeranyl diphosphate + 3-(O-geranylgeranyl)-sn-glycerol 1-phosphate Saccharolobus solfataricus the enzyme carries out the second prenyltransfer reaction involved in the formation of polar membrane lipids in archaea diphosphate + 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q9UWY6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranylgeranyl diphosphate + 3-(O-geranylgeranyl)-sn-glycerol 1-phosphate
-
 Saccharolobus solfataricus diphosphate + 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate
-
 ?
geranylgeranyl diphosphate + 3-(O-geranylgeranyl)-sn-glycerol 1-phosphate the enzyme carries out the second prenyltransfer reaction involved in the formation of polar membrane lipids in archaea Saccharolobus solfataricus diphosphate + 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate
-
 ?
additional information 4-hydroxybenzoate, 1,4-dihydroxy 2-naphthoate, homogentisate, and alpha-glycerophosphate cannot act as prenyl acceptor substrates Saccharolobus solfataricus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 28000, SDS-PAGE Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
(S)-2,3-di-O-geranylgeranylglyceryl phosphate synthase
-
 Saccharolobus solfataricus
DGGGP synthase
-
 Saccharolobus solfataricus
DGGGPS
-
 Saccharolobus solfataricus
UbiA-2 protein
-
 Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Saccharolobus solfataricus

General Information

General Information Comment Organism
physiological function the enzyme carries out the second prenyltransfer reaction involved in the formation of polar membrane lipids in archaea Saccharolobus solfataricus