General Stability | Organism |
---|---|
not stable to repeated freezing and thawing | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | - |
Escherichia coli | |
additional information | no effect: Mg2+, EDTA | Escherichia coli | |
S-adenosyl-L-ethionine | racemic mixture, 0.2 mM, 50% inhibition | Escherichia coli | |
S-adenosyl-L-homocysteine | - |
Escherichia coli | |
S-Ribosylmethionine | - |
Escherichia coli | |
SH-binding reagents | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
S-adenosyl-L-methionine | - |
Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
infected with bacteriophage T3 and UV-T3 | - |
Purification (Comment) | Organism |
---|---|
method that includes chromatography on DEAE-cellulose | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.07 | - |
E. coli infected with bacteriophage T3 | Escherichia coli |
28.3 | - |
E. coli infected with bacteriophage UV-T3 | Escherichia coli |
Storage Stability | Organism |
---|---|
-10°C, enzyme from E. coli infected with bacteriophage UV-T3 is stable for 2 weeks | Escherichia coli |
0°C or -10°C, enzyme from E. coli infected with bacteriophage T3 loses all of its activity within 3 days | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine | - |
Escherichia coli | 5'-methylthioadenosine + 2-aminobutan-4-olide | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | about 60% of activity maximum at pH 6 and 9 | Escherichia coli |