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Literature summary for 2.5.1.4 extracted from

  • Gefter, M.L.
    T3 and UV-T3-induced S-adenosylmethionine cleavage enzyme (Escherichia coli) (1971), Methods Enzymol., 17 B, 406-411.
No PubMed abstract available

General Stability

General Stability Organism
not stable to repeated freezing and thawing Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Mn2+
-
Escherichia coli
additional information no effect: Mg2+, EDTA Escherichia coli
S-adenosyl-L-ethionine racemic mixture, 0.2 mM, 50% inhibition Escherichia coli
S-adenosyl-L-homocysteine
-
Escherichia coli
S-Ribosylmethionine
-
Escherichia coli
SH-binding reagents
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
S-adenosyl-L-methionine
-
Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
infected with bacteriophage T3 and UV-T3
-

Purification (Commentary)

Purification (Comment) Organism
method that includes chromatography on DEAE-cellulose Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.07
-
E. coli infected with bacteriophage T3 Escherichia coli
28.3
-
E. coli infected with bacteriophage UV-T3 Escherichia coli

Storage Stability

Storage Stability Organism
-10°C, enzyme from E. coli infected with bacteriophage UV-T3 is stable for 2 weeks Escherichia coli
0°C or -10°C, enzyme from E. coli infected with bacteriophage T3 loses all of its activity within 3 days Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine
-
Escherichia coli 5'-methylthioadenosine + 2-aminobutan-4-olide
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
6 9 about 60% of activity maximum at pH 6 and 9 Escherichia coli