Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Brij W1 | inhibition at 0.5% or more, activation at 0.05% | Rattus norvegicus | |
Nonidet P-40 | inhibition at 0.5% or more, activation at 0.05% | Rattus norvegicus | |
sodium deoxycholate | inhibition at 0.5% or more, activation at 0.05% | Rattus norvegicus | |
Triton X-100 | inhibition at 0.5% or more, activation at 0.01% | Rattus norvegicus | |
Tween 80 | inhibition at 0.5% or more, activation at 0.01% | Rattus norvegicus |
General Stability | Organism |
---|---|
isolated mitochondria can be stored frozen for 2-3 weeks prior to preparation of membrane fragments without any loss of activity, preparation of mitochondrial fragments loses activity after 2 cycles of freezing and thawing | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-Aminobenzoic acid | - |
Rattus norvegicus | |
4-Chlorobenzoic acid | - |
Rattus norvegicus | |
bacitracin | strong inhibitor | Rattus norvegicus | |
Brij W1 | inhibition at 0.5% or more, activation at 0.05% | Rattus norvegicus | |
Ca2+ | Mg2+ can overcome the inhibition | Rattus norvegicus | |
Diphenylamine | - |
Rattus norvegicus | |
iodoacetamide | - |
Rattus norvegicus | |
KCN | - |
Rattus norvegicus | |
NaF | - |
Rattus norvegicus | |
Nonidet P-40 | inhibition at 0.5% or more, activation at 0.05% | Rattus norvegicus | |
p-chloromercuribenzoate | - |
Rattus norvegicus | |
sodium deoxycholate | inhibition at 0.5% or more, activation at 0.05% | Rattus norvegicus | |
Triton X-100 | inhibition at 0.5% or more, activation at 0.01% | Rattus norvegicus | |
Tween 80 | inhibition at 0.5% or more, activation at 0.01% | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000833 | - |
nonaprenyl diphosphate | pH 7.4, 25°C or 37°C | Rattus norvegicus | |
0.00114 | - |
4-hydroxybenzoate | pH 7.4, 25°C or 37°C | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial inner membrane | - |
Rattus norvegicus | 5743 | - |
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activation | Rattus norvegicus | |
Mg2+ | maximal activation: 10 mM | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
solanesyl diphosphate + 4-hydroxybenzoate | Rattus norvegicus | key enzyme in ubiquinone synthesis, it catalyzes the step which brings together the precursor of the benzoquinone ring and the polyisoprenoid side chain, in rat liver the major product is 3-nonaprenyl-4-hydroxybenzoate, in human and guinea pig it is 3-decaprenyl-4-hydroxybenzoate | diphosphate + 3-solanesyl-4-hydroxybenzoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
male Sprague-Dawley | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | - |
Rattus norvegicus | - |
kidney | - |
Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
solanesyl diphosphate + 4-hydroxybenzoate | i.e. nonaprenyl diphosphate | Rattus norvegicus | diphosphate + 3-solanesyl-4-hydroxybenzoate | small amounts of decaprenyl-4-hydroxybenzoate and octaprenylhydroxybenzoate can be found, depending on source of enzyme and substrate used to provide polyprenyl side chains | ? | |
solanesyl diphosphate + 4-hydroxybenzoate | key enzyme in ubiquinone synthesis, it catalyzes the step which brings together the precursor of the benzoquinone ring and the polyisoprenoid side chain, in rat liver the major product is 3-nonaprenyl-4-hydroxybenzoate, in human and guinea pig it is 3-decaprenyl-4-hydroxybenzoate | Rattus norvegicus | diphosphate + 3-solanesyl-4-hydroxybenzoate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Rattus norvegicus |