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Literature summary for 2.5.1.34 extracted from

  • Rilling, H.C.
    Dimethylallylpyrophosphate: L-tryptophan dimethylallyltransferase (1985), Methods Enzymol., 110, 335-340.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Claviceps sp.

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Claviceps sp.
2,2'-bipyridyl
-
Claviceps sp.
8-hydroxyquinoline
-
Claviceps sp.
8-mercaptoquinoline
-
Claviceps sp.
Acetylacetone
-
Claviceps sp.
Ca2+ 30 mM inhibits Claviceps sp.
Chelating agents in presence of excess of Ca2+ Claviceps sp.
dithiothreitol
-
Claviceps sp.
EDTA
-
Claviceps sp.
nitrilotriacetic acid
-
Claviceps sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Claviceps sp.
0.007
-
dimethylallyl diphosphate in presence of Ca2+ Claviceps sp.
0.009
-
tryptophan in presence of Ca2+ Claviceps sp.

Metals/Ions

Metals/Ions Comment Organism Structure
additional information enzyme contains a divalent cation which is most likely involved in catalysis Claviceps sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dimethylallyl diphosphate + L-tryptophan Claviceps sp. first pathway-specific enzyme of ergot alkaloid biosynthesis diphosphate + L-4-(gamma,gamma-dimethylallyl)tryptophan
-
?
dimethylallyl diphosphate + L-tryptophan Claviceps sp. SD58 first pathway-specific enzyme of ergot alkaloid biosynthesis diphosphate + L-4-(gamma,gamma-dimethylallyl)tryptophan
-
?

Organism

Organism UniProt Comment Textmining
Claviceps sp.
-
SD58
-
Claviceps sp. SD58
-
SD58
-

Purification (Commentary)

Purification (Comment) Organism
-
Claviceps sp.

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Claviceps sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.35
-
-
Claviceps sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate + L-tryptophan
-
Claviceps sp. diphosphate + L-4-(gamma,gamma-dimethylallyl)tryptophan
-
?
dimethylallyl diphosphate + L-tryptophan first pathway-specific enzyme of ergot alkaloid biosynthesis Claviceps sp. diphosphate + L-4-(gamma,gamma-dimethylallyl)tryptophan
-
?
dimethylallyl diphosphate + L-tryptophan
-
Claviceps sp. SD58 diphosphate + L-4-(gamma,gamma-dimethylallyl)tryptophan
-
?
dimethylallyl diphosphate + L-tryptophan first pathway-specific enzyme of ergot alkaloid biosynthesis Claviceps sp. SD58 diphosphate + L-4-(gamma,gamma-dimethylallyl)tryptophan
-
?