Crystallization (Comment) | Organism |
---|---|
- |
Claviceps sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Claviceps sp. | |
2,2'-bipyridyl | - |
Claviceps sp. | |
8-hydroxyquinoline | - |
Claviceps sp. | |
8-mercaptoquinoline | - |
Claviceps sp. | |
Acetylacetone | - |
Claviceps sp. | |
Ca2+ | 30 mM inhibits | Claviceps sp. | |
Chelating agents | in presence of excess of Ca2+ | Claviceps sp. | |
dithiothreitol | - |
Claviceps sp. | |
EDTA | - |
Claviceps sp. | |
nitrilotriacetic acid | - |
Claviceps sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Claviceps sp. | |
0.007 | - |
dimethylallyl diphosphate | in presence of Ca2+ | Claviceps sp. | |
0.009 | - |
tryptophan | in presence of Ca2+ | Claviceps sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme contains a divalent cation which is most likely involved in catalysis | Claviceps sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + L-tryptophan | Claviceps sp. | first pathway-specific enzyme of ergot alkaloid biosynthesis | diphosphate + L-4-(gamma,gamma-dimethylallyl)tryptophan | - |
? | |
dimethylallyl diphosphate + L-tryptophan | Claviceps sp. SD58 | first pathway-specific enzyme of ergot alkaloid biosynthesis | diphosphate + L-4-(gamma,gamma-dimethylallyl)tryptophan | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Claviceps sp. | - |
SD58 | - |
Claviceps sp. SD58 | - |
SD58 | - |
Purification (Comment) | Organism |
---|---|
- |
Claviceps sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Claviceps sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.35 | - |
- |
Claviceps sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + L-tryptophan | - |
Claviceps sp. | diphosphate + L-4-(gamma,gamma-dimethylallyl)tryptophan | - |
? | |
dimethylallyl diphosphate + L-tryptophan | first pathway-specific enzyme of ergot alkaloid biosynthesis | Claviceps sp. | diphosphate + L-4-(gamma,gamma-dimethylallyl)tryptophan | - |
? | |
dimethylallyl diphosphate + L-tryptophan | - |
Claviceps sp. SD58 | diphosphate + L-4-(gamma,gamma-dimethylallyl)tryptophan | - |
? | |
dimethylallyl diphosphate + L-tryptophan | first pathway-specific enzyme of ergot alkaloid biosynthesis | Claviceps sp. SD58 | diphosphate + L-4-(gamma,gamma-dimethylallyl)tryptophan | - |
? |