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Literature summary for 2.5.1.34 extracted from
- Purification and properties of dimethylallylpyrophosphate:tryptophan dimethylallyl transferase, the first enzyme of ergot alkaloid biosynthesis in Claviceps. sp. SD 58 (1976), Arch. Biochem. Biophys., 177, 84-94.
General Stability
| General Stability | Organism |
|---|---|
| repeated thawing and freezing greatly accelerates denaturation | Claviceps sp. |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | no inhibition, even at 5 mM | Claviceps sp. |  |
| Co3+ | - |
Claviceps sp. | |
| diphosphate | noncompetitive to dimethylallyl diphosphate | Claviceps sp. | |
| Hg2+ | - |
Claviceps sp. | |
| L-4-(gamma,gamma-dimethylallyl)tryptophan | product inhibition, competitive to dimethylallyl diphosphate, noncompetitive to L-tryptophan | Claviceps sp. | |
| Mg2+ | inhibition above 5 mM | Claviceps sp. | |
| Mn2+ | - |
Claviceps sp. | |
| Zn2+ | - |
Claviceps sp. | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.067 | - |
L-tryptophan | 30°C | Claviceps sp. | |
| 0.2 | - |
dimethylallyl diphosphate | 30°C | Claviceps sp. | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | partial activation | Claviceps sp. | |
| Cu2+ | slight stimulation | Claviceps sp. | |
| Fe2+ | activates | Claviceps sp. | |
| Li+ | slight stimulation | Claviceps sp. | |
| Mg2+ | activates | Claviceps sp. | |
| Mg2+ | optimal concentration: 3-5 mM | Claviceps sp. | |
| Na+ | slight stimulation | Claviceps sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
1 * 70000, SDS-PAGE | Claviceps sp. |
| 210000 | - |
gel filtration | Claviceps sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethylallyl diphosphate + L-tryptophan | Claviceps sp. | first pathway-specific enzyme of ergot alkaloid biosynthesis | diphosphate + L-4-(gamma,gamma-dimethylallyl)tryptophan | - |
? | |
| dimethylallyl diphosphate + L-tryptophan | Claviceps sp. SD58 | first pathway-specific enzyme of ergot alkaloid biosynthesis | diphosphate + L-4-(gamma,gamma-dimethylallyl)tryptophan | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Claviceps sp. | - |
SD58 | - |
| Claviceps sp. SD58 | - |
SD58 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Claviceps sp. |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| dimethylallyl diphosphate + L-tryptophan = diphosphate + 4-(3-methylbut-2-enyl)-L-tryptophan | sequential mechanism | Claviceps sp. |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Claviceps sp. | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.309 | - |
- |
Claviceps sp. |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 10 mM Tris-HCl, pH 8.0, 20 mM CaCl2, 20 mM 2-mercaptoethanol, 10% glycerol, 2 mg/ml enzyme concentration, stable for 1 week | Claviceps sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethylallyl diphosphate + 5-methyltryptophan | 7.4% of the activity with L-tryptophan | Claviceps sp. | diphosphate + 4-(3-methylbut-2-enyl)-5-methyl-L-tryptophan | - |
? | |
| dimethylallyl diphosphate + 5-methyltryptophan | 7.4% of the activity with L-tryptophan | Claviceps sp. SD58 | diphosphate + 4-(3-methylbut-2-enyl)-5-methyl-L-tryptophan | - |
? | |
| dimethylallyl diphosphate + 6-methyltryptophan | 9.8% of the activity with tryptophan | Claviceps sp. | diphosphate + 4-(3-methylbut-2-enyl)-6-methyl-L-tryptophan | - |
? | |
| dimethylallyl diphosphate + 6-methyltryptophan | 9.8% of the activity with tryptophan | Claviceps sp. SD58 | diphosphate + 4-(3-methylbut-2-enyl)-6-methyl-L-tryptophan | - |
? | |
| dimethylallyl diphosphate + 7-methyltryptophan | 40.5% of the activity with tryptophan | Claviceps sp. | diphosphate + 4-(3-methylbut-2-enyl)-7-methyl-L-tryptophan | - |
? | |
| dimethylallyl diphosphate + 7-methyltryptophan | 40.5% of the activity with tryptophan | Claviceps sp. SD58 | diphosphate + 4-(3-methylbut-2-enyl)-7-methyl-L-tryptophan | - |
? | |
| dimethylallyl diphosphate + bishomotryptophan | 2% of the activity with tryptophan | Claviceps sp. | diphosphate + bis[4-(3-methylbut-2-enyl)]-L-homotryptophan | - |
? | |
| dimethylallyl diphosphate + L-tryptophan | - |
Claviceps sp. | diphosphate + L-4-(gamma,gamma-dimethylallyl)tryptophan | - |
? | |
| dimethylallyl diphosphate + L-tryptophan | first pathway-specific enzyme of ergot alkaloid biosynthesis | Claviceps sp. | diphosphate + L-4-(gamma,gamma-dimethylallyl)tryptophan | - |
? | |
| dimethylallyl diphosphate + L-tryptophan | - |
Claviceps sp. SD58 | diphosphate + L-4-(gamma,gamma-dimethylallyl)tryptophan | - |
? | |
| dimethylallyl diphosphate + L-tryptophan | first pathway-specific enzyme of ergot alkaloid biosynthesis | Claviceps sp. SD58 | diphosphate + L-4-(gamma,gamma-dimethylallyl)tryptophan | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 70000, SDS-PAGE | Claviceps sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
1 h, complete loss of activity | Claviceps sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | - |
Claviceps sp. |
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