Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Tween 80 | detergent required, maximal activity at 0.08% | Pantoea agglomerans |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pantoea agglomerans |
Protein Variants | Comment | Organism |
---|---|---|
additional information | to facilitate purification of the recombinant enzyme, the structural gene for Psase is modified by site-directed mutagenesis to incorporate a C-terminal Glu-Glu-Phe tripeptide to allow purification by immunoaffinity chromatography on an immobilized monoclonal anti-alpha-tubulin antibody YL1/2 column | Pantoea agglomerans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | upon induction the recombinant enzyme constitutes 5-10% of the total soluble protein | Pantoea agglomerans | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required | Pantoea agglomerans | |
Mn2+ | maximal activity at 0.25 mM | Pantoea agglomerans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34500 | - |
x * 34500, recombinant enzyme, SDS-PAGE | Pantoea agglomerans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate + geranylgeranyl diphosphate | Pantoea agglomerans | first pathway-specific step in carotenoid biosynthesis | prephytoene diphosphate + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pantoea agglomerans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to facilitate purification of the recombinant enzyme, the structural gene for Psase is modified by site-directed mutagenesis to incorporate a C-terminal Glu-Glu-Phe tripeptide to allow purification by immunoaffinity chromatography on an immobilized monoclonal anti-alpha-tubulin antibody YL1/2 column | Pantoea agglomerans |
Storage Stability | Organism |
---|---|
flash frozen in liquid N2 and stored at -80°C, stable for at least 6 months | Pantoea agglomerans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate + geranylgeranyl diphosphate | the same enzyme catalyzes two consecutive reactions: 1. the synthesis of prephytoene diphosphate and 2. the synthesis of phytoene | Pantoea agglomerans | prephytoene diphosphate + diphosphate | - |
? | |
geranylgeranyl diphosphate + geranylgeranyl diphosphate | first pathway-specific step in carotenoid biosynthesis | Pantoea agglomerans | prephytoene diphosphate + diphosphate | - |
? | |
prephytoene diphosphate | the same enzyme catalyzes two consecutive reactions: 1. the synthesis of prephytoene diphosphate and 2. the synthesis of phytoene | Pantoea agglomerans | phytoene + diphosphate | 15,15'-Z-phytoene is the sole product | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 34500, recombinant enzyme, SDS-PAGE | Pantoea agglomerans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
- |
Pantoea agglomerans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.8 | 8.8 | about 70% of maximal activity at pH 6.8 and pH 8.8 | Pantoea agglomerans |