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Literature summary for 2.5.1.32 extracted from

  • Iwata-Reuyl, D.; Math, S.K.; Desai, S.B.; Poulter, C.D.
    Bacterial phytoene synthase: molecular cloning, expression, and characterization of Erwinia herbicola phytoene synthase (2003), Biochemistry, 42, 3359-3365.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Tween 80 detergent required, maximal activity at 0.08% Pantoea agglomerans

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pantoea agglomerans

Protein Variants

Protein Variants Comment Organism
additional information to facilitate purification of the recombinant enzyme, the structural gene for Psase is modified by site-directed mutagenesis to incorporate a C-terminal Glu-Glu-Phe tripeptide to allow purification by immunoaffinity chromatography on an immobilized monoclonal anti-alpha-tubulin antibody YL1/2 column Pantoea agglomerans

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble upon induction the recombinant enzyme constitutes 5-10% of the total soluble protein Pantoea agglomerans
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ required Pantoea agglomerans
Mn2+ maximal activity at 0.25 mM Pantoea agglomerans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34500
-
x * 34500, recombinant enzyme, SDS-PAGE Pantoea agglomerans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranylgeranyl diphosphate + geranylgeranyl diphosphate Pantoea agglomerans first pathway-specific step in carotenoid biosynthesis prephytoene diphosphate + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Pantoea agglomerans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to facilitate purification of the recombinant enzyme, the structural gene for Psase is modified by site-directed mutagenesis to incorporate a C-terminal Glu-Glu-Phe tripeptide to allow purification by immunoaffinity chromatography on an immobilized monoclonal anti-alpha-tubulin antibody YL1/2 column Pantoea agglomerans

Storage Stability

Storage Stability Organism
flash frozen in liquid N2 and stored at -80°C, stable for at least 6 months Pantoea agglomerans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranylgeranyl diphosphate + geranylgeranyl diphosphate the same enzyme catalyzes two consecutive reactions: 1. the synthesis of prephytoene diphosphate and 2. the synthesis of phytoene Pantoea agglomerans prephytoene diphosphate + diphosphate
-
?
geranylgeranyl diphosphate + geranylgeranyl diphosphate first pathway-specific step in carotenoid biosynthesis Pantoea agglomerans prephytoene diphosphate + diphosphate
-
?
prephytoene diphosphate the same enzyme catalyzes two consecutive reactions: 1. the synthesis of prephytoene diphosphate and 2. the synthesis of phytoene Pantoea agglomerans phytoene + diphosphate 15,15'-Z-phytoene is the sole product ?

Subunits

Subunits Comment Organism
? x * 34500, recombinant enzyme, SDS-PAGE Pantoea agglomerans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
-
Pantoea agglomerans

pH Range

pH Minimum pH Maximum Comment Organism
6.8 8.8 about 70% of maximal activity at pH 6.8 and pH 8.8 Pantoea agglomerans