Activating Compound | Comment | Organism | Structure |
---|---|---|---|
galactose | or galactose moieties in galactolipids are required | Narcissus pseudonarcissus |
Cloned (Comment) | Organism |
---|---|
overexpressed in insect Sf9 cells using the baculovirus lipofection system. The full-length overexpressed enzyme exhibits very reduced catalytic activity compared with an overexpressed N-truncated form, with its transit sequence of 214 base pairs removed from the psy cDNA at the 5'-end by site-directed mutagenesis. The shortened form readily binds to lipid bilayers | Narcissus pseudonarcissus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the full-length overexpressed enzyme exhibits very reduced catalytic activity compared with an overexpressed N-truncated form, with its transit sequence of 214 base pairs removed from the psy cDNA at the 5'-end by site-directed mutagenesis. The shortened form readily binds to lipid bilayers | Narcissus pseudonarcissus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
fructose | - |
Narcissus pseudonarcissus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chromoplast | - |
Narcissus pseudonarcissus | 9509 | - |
membrane | the soluble enzyme is complexed and inactive, the membrane-bound enzyme is active | Narcissus pseudonarcissus | 16020 | - |
soluble | the soluble enzyme is complexed and inactive, the membrane-bound enzyme is active | Narcissus pseudonarcissus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | stimulatory in presence of Mn2+ | Narcissus pseudonarcissus | |
Mn2+ | absolute requirement, optimum at 1 mM | Narcissus pseudonarcissus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Narcissus pseudonarcissus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Narcissus pseudonarcissus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | inner corona of, in all stages the steady-state phytoene synthase transcript levels are low | Narcissus pseudonarcissus | - |
additional information | more activity in green leaves | Narcissus pseudonarcissus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate + geranylgeranyl diphosphate | - |
Narcissus pseudonarcissus | prephytoene diphosphate + diphosphate | - |
? |