Literature summary for 2.5.1.32 extracted from

Misawa, N.; Truesdale, M.R.; Sandmann, G.; Fraser, P.D.; Bird, C.; Schuch, W.; Bramley, P.M.
Expression of a tomato cDNA coding for phytoene synthase in Escherichia coli, phytoene formation in vivo and in vitro, and functional analysis of the various truncated gene products (1994), J. Biochem., 116, 980-985.

Search for more literature for 2.5.1.32

Cloned(Commentary)

Cloned (Comment)	Organism
full length and truncated cDNA expression constructs of the phytoene synthase gene from tomato ligated into a pUC8 cloning vector, expression in Escherichia coli carrying Erwinia uredovora geranylgeranyl diphosphate synthase gene	Solanum lycopersicum

Protein Variants

Protein Variants	Comment	Organism
additional information	the size of the transit peptide of phytoene synthase from ripe fruit is approximatyle 9000 Da, corresponding to about 80 amino acid residues. Removal of further N-terminal amino acids up to 114 from the enzyme, to yield a protein of apparent molecular mass 3400 Da, increases its catalytic activity in Escherichia coli	Solanum lycopersicum

Organism

Organism	UniProt	Comment	Textmining
Solanum lycopersicum	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	the size of the transit peptide of phytoene synthase from ripe fruit is approximatyle 9000 Da, corresponding to about 80 amino acid residues	Solanum lycopersicum

Source Tissue

Source Tissue	Comment	Organism	Textmining
fruit	-	Solanum lycopersicum	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
geranylgeranyl diphosphate + geranylgeranyl diphosphate	-	Solanum lycopersicum	prephytoene diphosphate + diphosphate	-	?

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Release 2023.1 (January 2023)