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Literature summary for 2.5.1.30 extracted from
- Heptaprenylpyrophosphate synthetase from Bacillus subtilis (1985), Methods Enzymol., 110, 199-205.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0083 | - |
geranylgeranyl diphosphate | pH 7.5, 37°C | Bacillus subtilis |  |
| 0.0128 | - |
isopentenyl diphosphate | pH 7.5, 37°C | Bacillus subtilis | |
| 0.0133 | - |
(2E,6E)-farnesyl diphosphate | pH 7.5, 37°C | Bacillus subtilis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | absolute requirement | Bacillus subtilis | |
| Mn2+ | 30% of the activation obtained with Mg2+ | Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
molecular weight of enzyme component I and enzyme component II is both about 30000 Da, gel filtration | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate | Bacillus subtilis | synthesis of all-trans-heptaprenyl diphosphate, which is precursor for the side chain of menaquinone-7 | 4 diphosphate + all-trans-heptaprenyl diphosphate | - |
? | |
| (2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate | Bacillus subtilis PCI-219 | synthesis of all-trans-heptaprenyl diphosphate, which is precursor for the side chain of menaquinone-7 | 4 diphosphate + all-trans-heptaprenyl diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | P31112 and P31114 | P31112: subunit 1, P31114: subunit 2; PCI-219 | - |
| Bacillus subtilis PCI-219 | P31112 and P31114 | P31112: subunit 1, P31114: subunit 2; PCI-219 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| separation of component I and component II | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate | - |
Bacillus subtilis | 4 diphosphate + all-trans-heptaprenyl diphosphate | - |
? | |
| (2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate | synthesis of all-trans-heptaprenyl diphosphate, which is precursor for the side chain of menaquinone-7 | Bacillus subtilis | 4 diphosphate + all-trans-heptaprenyl diphosphate | - |
? | |
| (2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate | - |
Bacillus subtilis PCI-219 | 4 diphosphate + all-trans-heptaprenyl diphosphate | - |
? | |
| (2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate | synthesis of all-trans-heptaprenyl diphosphate, which is precursor for the side chain of menaquinone-7 | Bacillus subtilis PCI-219 | 4 diphosphate + all-trans-heptaprenyl diphosphate | - |
? | |
| geranylgeranyl diphosphate + 3 isopentenyl diphosphate | - |
Bacillus subtilis | 3 diphosphate + all-trans-heptaprenyl diphosphate | - |
? | |
| geranylgeranyl diphosphate + 3 isopentenyl diphosphate | - |
Bacillus subtilis PCI-219 | 3 diphosphate + all-trans-heptaprenyl diphosphate | - |
? | |
| additional information | neither dimethylallyl diphosphate nor geranyl diphosphate is active as primer | Bacillus subtilis | ? | - |
? | |
| additional information | neither dimethylallyl diphosphate nor geranyl diphosphate is active as primer | Bacillus subtilis PCI-219 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | molecular weight of enzyme component I and enzyme component II is both about 30000 Da, gel filtration | Bacillus subtilis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| heptaprenylpyrophosphate synthetase | - |
Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
component II is inactivated almost completely within 5 min, component II does not lose activity within 30 min | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7 | - |
Bacillus subtilis |
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