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Literature summary for 2.5.1.29 extracted from

  • Hirooka, K.; Kato, T.; Matsu-ura, J.; Hemmi, H.; Nishino, T.
    The role of histidine-114 of Sulfolobus acidocaldarius geranylgeranyl diphosphate synthase in chain-length determination (2000), FEBS Lett., 481, 68-72.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
F77G C30-prenyl diphosphate is the main long product Sulfolobus acidocaldarius
F77G/H114A C45-prenyl diphosphate is the main long product Sulfolobus acidocaldarius
F77G/H114G although its activity is approximately 2-5% of the wild-type, this double-mutated enzyme exhibits a detectable activity and a thermostability to resist 15 h of heat treatment at 55°C. C50-prenyl diphosphate is the main long product Sulfolobus acidocaldarius
H114A C30-prenyl diphosphate is the main long product Sulfolobus acidocaldarius
H114G C40-prenyl diphosphate is the main long product Sulfolobus acidocaldarius

Organism

Organism UniProt Comment Textmining
Sulfolobus acidocaldarius
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate the wild-type enzyme produces mostly 87% geranylgeranyl diphosphate. The mutant enzymes F77G, F77G/H114A, F77G/H114G, H114A, and H114G give C30, C45, C50, C30 and C40 as the main long product, respectively Sulfolobus acidocaldarius diphosphate + geranylgeranyl diphosphate
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Synonyms

Synonyms Comment Organism
GGPS
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Sulfolobus acidocaldarius

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
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assay at Sulfolobus acidocaldarius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
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assay at Sulfolobus acidocaldarius