Literature summary for 2.5.1.29 extracted from

Kavanagh, K.L.; Dunford, J.E.; Bunkoczi, G.; Russell, R.G.; Oppermann, U.
The crystal structure of human geranylgeranyl pyrophosphate synthase reveals a novel hexameric arrangement and inhibitory product binding (2006), J. Biol. Chem., 281, 22004-22012.

Activating Compound

Activating Compound	Comment	Organism	Structure
Tween 20	at 0.2%	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expression of N-terminally His-tagged enzyme in Escherichia coli	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
native protein, 20°C, sitting drop vapour diffusion mthod, mixing of 200 nl of 90 mg/ml protein in 10 mM HEPES, pH 7.5, 500 mM NaCl, 5% glycerol with 100 nl of precipitant solution consisting of 25% PEG 3350, 200 mM magnesium formate, pH 5.5, and equilibration against 0.1 ml of the precipitant solution, selenomethionine-labeled protein by suspending a 0.003 ml drop containing 26 mg/ml protein, 333 mM NaCl, 0.67 mM MgCl2, 0.67 mM GGPP, 3% glycerol, 15% 2-methyl-2,4-pentanediol, 1.7% PEG 10,000, 6.7 mM HEPES, pH 7.5, over a 1 ml reservoir containing 45% 2-methyl-2,4-pentanediol, and 5% PEG 10000, X-ray diffraction structure determination and analysis at 2.7-2.8 A resolution	Homo sapiens

Crystallization (Comment)

Organism

native protein, 20°C, sitting drop vapour diffusion mthod, mixing of 200 nl of 90 mg/ml protein in 10 mM HEPES, pH 7.5, 500 mM NaCl, 5% glycerol with 100 nl of precipitant solution consisting of 25% PEG 3350, 200 mM magnesium formate, pH 5.5, and equilibration against 0.1 ml of the precipitant solution, selenomethionine-labeled protein by suspending a 0.003 ml drop containing 26 mg/ml protein, 333 mM NaCl, 0.67 mM MgCl2, 0.67 mM GGPP, 3% glycerol, 15% 2-methyl-2,4-pentanediol, 1.7% PEG 10,000, 6.7 mM HEPES, pH 7.5, over a 1 ml reservoir containing 45% 2-methyl-2,4-pentanediol, and 5% PEG 10000, X-ray diffraction structure determination and analysis at 2.7-2.8 A resolution

Homo sapiens

Inhibitors

Inhibitors	Comment	Organism
EDTA	-	Homo sapiens
geranylgeranyl diphosphate	competitive product inhibition	Homo sapiens
nitrogen-containing bisphosphonates	-	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
additional information	-	additional information	steady-state kinetics, recombinant enzyme	Homo sapiens
0.003	-	isopentenyl diphosphate	pH 7.7, 37°C, recombinant enzyme	Homo sapiens
0.0042	-	trans,trans-farnesyl diphosphate	pH 7.7, 37°C, recombinant enzyme	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
200000	-	about, gel filtration	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
trans,trans-farnesyl diphosphate + isopentenyl diphosphate	Homo sapiens	the enzyme takes part in the mevalonate pathway of isoprenoid synthesis, and plays a crucial role in cell survival, overview	diphosphate + geranylgeranyl diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant N-terminally His-tagged enzyme from Escherichia coli, the His-tag is cleaved of by TEV	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
trans,trans-farnesyl diphosphate + isopentenyl diphosphate	the enzyme takes part in the mevalonate pathway of isoprenoid synthesis, and plays a crucial role in cell survival, overview	Homo sapiens	diphosphate + geranylgeranyl diphosphate	-	?
trans,trans-farnesyl diphosphate + isopentenyl diphosphate	tight binding substrate and product structures, overview	Homo sapiens	diphosphate + geranylgeranyl diphosphate	-	?

Subunits

Subunits	Comment	Organism
hexamer	three dimers join together to form a propeller-bladed hexameric molecule, quaternary structure, overview	Homo sapiens

Synonyms

Synonyms	Comment	Organism
geranylgeranyl pyrophosphate synthase	-	Homo sapiens
GGPS	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.204	-	trans,trans-farnesyl diphosphate	pH 7.7, 37°C, recombinant enzyme	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.7	-	assay at	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.025	-	geranylgeranyl diphosphate	pH 7.7, 37°C, recombinant enzyme, versus trans,trans-farnesyl diphosphate	Homo sapiens