Literature summary for 2.5.1.29 extracted from

Kloer, D.P.; Welsch, R.; Beyer, P.; Schulz, G.E.
Structure and reaction geometry of geranylgeranyl diphosphate synthase from Sinapis alba (2006), Biochemistry, 45, 15197-15204.

Cloned(Commentary)

Cloned (Comment)	Organism
expression of the His-tagged nezym ein Escherichia coli strain BL21(DE3)	Sinapis alba

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant wild-type of selenomethionine-labeled enzyme, 20 mg/mL protein in 10 mM Tris-HCl, pH 7.9, 10 mM MgCl2, 100 mM NaCl, and 0.5 mM tris(2-carboxyethyl)phosphine, crystals of SeMet-labeled enzyme are obtained by vapor diffusion with hanging drops that contain a 1:1 mixture of protein solution and reservoir buffer D, the latter containing 100 mM sodium acetate, pH 4.4, 40% v/v propanediol, 10 mM MgCl2, and 0.5 mM TCEP, liquid nitrogen freezing without the addition of a cryo protectant, a second crystal form is obtained with native enzyme using the same procedure but with reservoir buffer E containing 100 mM N-cyclohexyl-3-aminopropane sulfonic acid, pH 10.0, 200 mM NaCl, 9-11% PEG 8000, 10 mM MgCl2, and 0.5 mM TCEP, mixing with reservoir buffer E containing 30% v/v glycerol and flash-freezing in liquid nitrogen, X-ray diffraction structure determination and analysis at 1.8-2.0 A resolution, multiwavelength anomalous diffraction, soaking of native enzyme with FPP and Mg2+ fails, overview	Sinapis alba

Crystallization (Comment)

Organism

purified recombinant wild-type of selenomethionine-labeled enzyme, 20 mg/mL protein in 10 mM Tris-HCl, pH 7.9, 10 mM MgCl2, 100 mM NaCl, and 0.5 mM tris(2-carboxyethyl)phosphine, crystals of SeMet-labeled enzyme are obtained by vapor diffusion with hanging drops that contain a 1:1 mixture of protein solution and reservoir buffer D, the latter containing 100 mM sodium acetate, pH 4.4, 40% v/v propanediol, 10 mM MgCl2, and 0.5 mM TCEP, liquid nitrogen freezing without the addition of a cryo protectant, a second crystal form is obtained with native enzyme using the same procedure but with reservoir buffer E containing 100 mM N-cyclohexyl-3-aminopropane sulfonic acid, pH 10.0, 200 mM NaCl, 9-11% PEG 8000, 10 mM MgCl2, and 0.5 mM TCEP, mixing with reservoir buffer E containing 30% v/v glycerol and flash-freezing in liquid nitrogen, X-ray diffraction structure determination and analysis at 1.8-2.0 A resolution, multiwavelength anomalous diffraction, soaking of native enzyme with FPP and Mg2+ fails, overview

Sinapis alba

Inhibitors

Inhibitors	Comment	Organism	Structure
nitrogen-containing bisphosphonates	bind to the A-site, structure, overview	Sinapis alba

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	the enzyme structure contains two DDxxD motifs and three Mg2+ ions	Sinapis alba

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
trans,trans-farnesyl diphosphate + isopentenyl diphosphate	Sinapis alba	-	diphosphate + geranylgeranyl diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Sinapis alba	Q43133	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by cobalt affinity chromatography	Sinapis alba

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
trans,trans-farnesyl diphosphate + isopentenyl diphosphate	-	Sinapis alba	diphosphate + geranylgeranyl diphosphate	-	?
trans,trans-farnesyl diphosphate + isopentenyl diphosphate	-	Sinapis alba	diphosphate + geranylgeranyl diphosphate	enzyme-product complex structure	?

Subunits

Subunits	Comment	Organism
More	the enzyme structure harbors eleven alpha-helices typical for the FPP synthase family enzymes, the enzyme structure contains two DDxxD motifs and three Mg2+ ions	Sinapis alba

Synonyms

Synonyms	Comment	Organism
geranylgeranyl diphosphate synthase	-	Sinapis alba
GGPP synthase	-	Sinapis alba
More	the enzyme belongs to the FPP synthase family	Sinapis alba