Cloned (Comment) | Organism |
---|---|
expression of preprotein and truncated versions in Escherichia coli | Taxus canadensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0026 | - |
farnesyl diphosphate | - |
Taxus canadensis | |
0.0034 | - |
Mg2+ | - |
Taxus canadensis | |
0.0077 | - |
geranyl diphosphate | - |
Taxus canadensis | |
0.039 | - |
isopentenyl diphosphate | cosubstrate farnesyl diphosphate | Taxus canadensis | |
0.088 | - |
isopentenyl diphosphate | cosubstrate dimethylallyl diphosphate | Taxus canadensis | |
0.122 | - |
isopentenyl diphosphate | cosubstrate geranyl diphosphate | Taxus canadensis | |
0.127 | - |
dimethylallyl diphosphate | - |
Taxus canadensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
2 * 32000, recombinant enzyme, SDS-PAGE | Taxus canadensis |
66000 | - |
gel filtration | Taxus canadensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E,E)-farnesyl diphosphate + isopentenyl diphosphate | Abies grandis | - |
geranylgeranyl diphosphate + diphosphate | - |
? | |
dimethylallyl diphosphate + 3 isopentenyl diphosphate | Abies grandis | - |
geranylgeranyl diphosphate + 3 diphosphate | - |
? | |
geranyl diphosphate + 2 isopentenyl diphosphate | Abies grandis | - |
geranylgeranyl diphosphate + 2 diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Abies grandis | Q8W1R9 | - |
- |
Taxus canadensis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E,E)-farnesyl diphosphate + isopentenyl diphosphate | - |
Abies grandis | geranylgeranyl diphosphate + diphosphate | - |
? | |
dimethylallyl diphosphate + 3 isopentenyl diphosphate | - |
Taxus canadensis | geranylgeranyl diphosphate + 3 diphosphate | - |
? | |
dimethylallyl diphosphate + 3 isopentenyl diphosphate | - |
Abies grandis | geranylgeranyl diphosphate + 3 diphosphate | - |
? | |
farnesyl diphosphate + isopentenyl diphosphate | - |
Taxus canadensis | geranylgeranyl diphosphate + diphosphate | - |
? | |
geranyl diphosphate + 2 isopentenyl diphosphate | - |
Abies grandis | geranylgeranyl diphosphate + 2 diphosphate | - |
? | |
geranyl diphosphate + isopentenyl diphosphate | - |
Taxus canadensis | farnesyl diphosphate + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 32000, recombinant enzyme, SDS-PAGE | Taxus canadensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
dimethylallyl diphosphate | - |
Taxus canadensis | |
2.1 | - |
geranyl diphosphate | - |
Taxus canadensis | |
3.3 | - |
farnesyl diphosphate | - |
Taxus canadensis |