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Literature summary for 2.5.1.29 extracted from

  • Hefner, J.; Ketchum, R.E.B.; Croteau, R.
    Cloning and functional expression of a cDNA encoding geranylgeranyl diphosphate synthase from Taxus canadensis and assessment of the role of this prenyltransferase in cells induced for Taxol production (1998), Arch. Biochem. Biophys., 360, 62-74.
    View publication on PubMed
Search for more literature for 2.5.1.29

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Taxus canadensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
 farnesyl diphosphate recombinant enzyme Taxus canadensis
0.007
-
 isopentenyl diphosphate recombinant enzyme Taxus canadensis
0.035
-
 Mg2+ recombinant enzyme Taxus canadensis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Taxus canadensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31500
-
 2 * 31500, deduced from nucleotide sequence Taxus canadensis
60000
-
 recombinant truncated enzyme, gel filtration Taxus canadensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(E,E)-farnesyl diphosphate + isopentenyl diphosphate Taxus canadensis 66% of activity with geranyl diphosphate geranylgeranyl diphosphate + diphosphate
-
 ?
dimethylallyl diphosphate + 3 isopentenyl diphosphate Taxus canadensis 33% of activity with geranyl diphosphate geranylgeranyl diphosphate + 3 diphosphate
-
 ?
geranyl diphosphate + 2 isopentenyl diphosphate Taxus canadensis preferred substrate geranylgeranyl diphosphate + 2 diphosphate
-
 ?
geranyl diphosphate + 2 isopentenyl diphosphate Taxus canadensis involved in taxol biosynthesis geranylgeranyl diphosphate + 2 diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Taxus canadensis Q9ZPM3 canadian yew
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Taxus canadensis

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
 Taxus canadensis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E,E)-farnesyl diphosphate + isopentenyl diphosphate 66% of activity with geranyl diphosphate Taxus canadensis geranylgeranyl diphosphate + diphosphate
-
 ?
dimethylallyl diphosphate + 3 isopentenyl diphosphate 33% of activity with geranyl diphosphate Taxus canadensis geranylgeranyl diphosphate + 3 diphosphate
-
 ?
geranyl diphosphate + 2 isopentenyl diphosphate preferred substrate Taxus canadensis geranylgeranyl diphosphate + 2 diphosphate
-
 ?
geranyl diphosphate + 2 isopentenyl diphosphate involved in taxol biosynthesis Taxus canadensis geranylgeranyl diphosphate + 2 diphosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 31500, deduced from nucleotide sequence Taxus canadensis