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Literature summary for 2.5.1.29 extracted from

  • Ohnuma, S.i.; Hemmi, H.; Koyama, T.; Ogura, K.; Nishino, T.
    Recognition of allylic substrates in Sulfolobus acidocaldarius geranylgeranyl diphosphate synthase: analysis using mutated enzymes and artificial allylic substrates (1998), J. Biochem., 123, 1036-1040.
    View publication on PubMed
Search for more literature for 2.5.1.29

Protein Variants

Protein Variants Comment Organism
D170E/M171P/I172A/S173M no activity with dimethylallyl diphosphate, low activity with geranyl diphosphate Sulfolobus acidocaldarius
D170E/M171P/I172A/S173M mutant enzyme shows considerably lower activity toward primer substrates with a short prenyl chain, DMAPP and GPP, than the wild-type enzyme Sulfolobus acidocaldarius
D170E/M171P/I172A/S173R low activity with dimethylallyl diphosphate, approx. 60% of wild-type activity with geranyl diphosphate Sulfolobus acidocaldarius
D170E/M171P/I172R/S173V no activity with dimethylallyl diphosphate, low activity with geranyl diphosphate Sulfolobus acidocaldarius
D170E/M171P/I172R/S173V mutant enzyme shows considerably lower activity toward primer substrates with a short prenyl chain, DMAPP and GPP, than the wild-type enzyme. Scarcely accepts DMAPP as an allylic substrate. The mutant enzyme can not catalyze condensation of 3,7,ll-trimethyl-2,6,10,14-heptadecatetraenyl diphosphate with isopentenyl diphosphate. Does not accept short allylic substrates but forms products with the same length as those of the wild-type enzyme Sulfolobus acidocaldarius
M171P low activity with dimethylallyl diphosphate, approx. 50% of wild-type activity with geranyl diphosphate Sulfolobus acidocaldarius
M171P/I172R no activity with dimethylallyl diphosphate, low activity with geranyl diphosphate Sulfolobus acidocaldarius
M171P/S173V very low activity with dimethylallyl diphosphate, approx. 50% of wild-type activity with geranyl diphosphate Sulfolobus acidocaldarius

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranyl diphosphate + 2 isopentenyl diphosphate Sulfolobus acidocaldarius
-
 geranylgeranyl diphosphate + 2 diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Sulfolobus acidocaldarius
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E)-3,7-dimethyl-2-octenyl diphosphate + isopentenyl diphosphate 62% of activity with geranyl diphosphate Sulfolobus acidocaldarius ?
-
 ?
(2E)-3-methyl-2,6-heptadienyl diphosphate + isopentenyl diphosphate 24% of activity with geranyl diphosphate Sulfolobus acidocaldarius ?
-
 ?
(2E)-3-methyl-2-butenyl diphosphate + isopentenyl diphosphate 21.5% of activity with geranyl diphosphate Sulfolobus acidocaldarius ?
-
 ?
(2E)-3-methyl-2-hexenyl diphosphate + isopentenyl diphosphate 95% of activity with geranyl diphosphate Sulfolobus acidocaldarius ?
-
 ?
(2E)-3-methyl-2-pentenyl diphosphate + isopentenyl diphosphate 63% of activity with geranyl diphosphate Sulfolobus acidocaldarius ?
-
 ?
(E,E)-farnesyl diphosphate + isopentenyl diphosphate
-
 Sulfolobus acidocaldarius diphosphate + geranylgeranyl diphosphate
-
 ?
dimethylallyl diphosphate + 3 isopentenyl diphosphate
-
 Sulfolobus acidocaldarius geranylgeranyl diphosphate + 3 diphosphate
-
 ?
geranyl diphosphate + 2 isopentenyl diphosphate
-
 Sulfolobus acidocaldarius geranylgeranyl diphosphate + 2 diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
geranylgeranyl diphosphate synthase
-
 Sulfolobus acidocaldarius