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Literature summary for 2.5.1.29 extracted from
- Purification and properties of geranylgeranyl-diphosphate synthase from bovine brain (1994), J. Biol. Chem., 269, 20561-20566.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| octyl glucoside | 0.8%, 1.2fold activation | Bos taurus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (E,E,E)-geranylgeranyl diphosphate | 0.005 mM, 50% inhibition, competitive vs. farnesyl diphosphate | Bos taurus | |
| (Z,E,E)-Geranylgeranyl diphosphate | 0.005 mM, approx. 50% inhibition, competitive vs. farnesyl diphosphate | Bos taurus | |
| 3-azageranylgeranyl diphosphate | - |
Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00074 | - |
farnesyl diphosphate | - |
Bos taurus | |
| 0.0008 | - |
geranyl diphosphate | - |
Bos taurus | |
| 0.002 | - |
isopentenyl diphosphate | - |
Bos taurus | |
| 0.033 | - |
dimethylallyl diphosphate | - |
Bos taurus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | maximal activity at 1 mM | Bos taurus | |
| Mg2+ | required for activity | Bos taurus | |
| Mn2+ | required for activity | Bos taurus | |
| Mn2+ | maximal activity at 1 mM, less effective than Mg2+ | Bos taurus | |
| Zn2+ | maximal activity at 1 mM, approx. 30% of activation with Mg2+ | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37500 | - |
x * 37500, tetramer or pentamer, SDS-PAGE | Bos taurus |
| 150000 | - |
Superose 12, gel filtration | Bos taurus |
| 190000 | - |
Superdex 200, gel filtration | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (E,E)-farnesyl diphosphate + isopentenyl diphosphate | Bos taurus | - |
geranylgeranyl diphosphate + diphosphate | - |
? | |
| geranyl diphosphate + 2 isopentenyl diphosphate | Bos taurus | - |
geranylgeranyl diphosphate + 2 diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, farnesylmethyl affinity gel | Bos taurus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.098 | - |
- |
Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (E,E)-farnesyl diphosphate + isopentenyl diphosphate | - |
Bos taurus | geranylgeranyl diphosphate + diphosphate | - |
? | |
| (E,E)-farnesyl diphosphate + isopentenyl diphosphate | - |
Bos taurus | geranylgeranyl diphosphate + diphosphate | exclusive product | ? | |
| dimethylallyl diphosphate + 3 isopentenyl diphosphate | - |
Bos taurus | geranylgeranyl diphosphate + 3 diphosphate | - |
? | |
| geranyl diphosphate + 2 isopentenyl diphosphate | - |
Bos taurus | geranylgeranyl diphosphate + 2 diphosphate | - |
? | |
| geranyl diphosphate + 2 isopentenyl diphosphate | - |
Bos taurus | geranylgeranyl diphosphate + 2 diphosphate | geranylgeranyl diphosphate + farnesyl diphosphate | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 37500, tetramer or pentamer, SDS-PAGE | Bos taurus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0012 | - |
(Z,E,E)-Geranylgeranyl diphosphate | - |
Bos taurus | |
| 0.0012 | - |
(E,E,E)-geranylgeranyl diphosphate | - |
Bos taurus | |
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