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Literature summary for 2.5.1.29 extracted from

  • Tachibana, A.; Tanaka, T.; Taniguchi, M.; Oi, S.
    Purification and characterization of geranylgeranyl diphosphate synthase from methanobacterium thermoformicicum Sf-4 (1993), Biosci. Biotechnol. Biochem., 57, 1129-1133.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
diphosphate 10 mM, 97% inhibition Methanothermobacter thermautotrophicus
p-chloromercuribenzoate 1 mM, 90% inhibition, 30% inhibition in the presence of 800 mM KCl Methanothermobacter thermautotrophicus
SDS 1%, 97% inhibition Methanothermobacter thermautotrophicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0126
-
geranyl diphosphate
-
Methanothermobacter thermautotrophicus
0.0147
-
farnesyl diphosphate
-
Methanothermobacter thermautotrophicus
0.0168
-
dimethylallyl diphosphate
-
Methanothermobacter thermautotrophicus
0.0308
-
isopentenyl diphosphate
-
Methanothermobacter thermautotrophicus

Metals/Ions

Metals/Ions Comment Organism Structure
KCl 800 mM, activates Methanothermobacter thermautotrophicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
native PAGE Methanothermobacter thermautotrophicus
78000
-
gel filtration Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(E,E)-farnesyl diphosphate + isopentenyl diphosphate Methanothermobacter thermautotrophicus
-
geranylgeranyl diphosphate + diphosphate
-
?
(E,E)-farnesyl diphosphate + isopentenyl diphosphate Methanothermobacter thermautotrophicus SF-4
-
geranylgeranyl diphosphate + diphosphate
-
?
dimethylallyl diphosphate + 3 isopentenyl diphosphate Methanothermobacter thermautotrophicus
-
geranylgeranyl diphosphate + 3 diphosphate
-
?
dimethylallyl diphosphate + 3 isopentenyl diphosphate Methanothermobacter thermautotrophicus SF-4
-
geranylgeranyl diphosphate + 3 diphosphate
-
?
geranyl diphosphate + 2 isopentenyl diphosphate Methanothermobacter thermautotrophicus
-
geranylgeranyl diphosphate + 2 diphosphate
-
?
geranyl diphosphate + 2 isopentenyl diphosphate Methanothermobacter thermautotrophicus SF-4
-
geranylgeranyl diphosphate + 2 diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Methanothermobacter thermautotrophicus
-
SF-4
-
Methanothermobacter thermautotrophicus SF-4
-
SF-4
-

Purification (Commentary)

Purification (Comment) Organism
-
Methanothermobacter thermautotrophicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.14
-
with farnesyl diphosphate Methanothermobacter thermautotrophicus
0.289
-
with geranyl diphosphate Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E,E)-farnesyl diphosphate + isopentenyl diphosphate
-
Methanothermobacter thermautotrophicus geranylgeranyl diphosphate + diphosphate
-
?
(E,E)-farnesyl diphosphate + isopentenyl diphosphate
-
Methanothermobacter thermautotrophicus SF-4 geranylgeranyl diphosphate + diphosphate
-
?
dimethylallyl diphosphate + 3 isopentenyl diphosphate
-
Methanothermobacter thermautotrophicus geranylgeranyl diphosphate + 3 diphosphate
-
?
dimethylallyl diphosphate + 3 isopentenyl diphosphate
-
Methanothermobacter thermautotrophicus SF-4 geranylgeranyl diphosphate + 3 diphosphate
-
?
geranyl diphosphate + 2 isopentenyl diphosphate
-
Methanothermobacter thermautotrophicus geranylgeranyl diphosphate + 2 diphosphate
-
?
geranyl diphosphate + 2 isopentenyl diphosphate
-
Methanothermobacter thermautotrophicus geranylgeranyl diphosphate + 2 diphosphate geranyl diphosphate + farnesyl diphosphate, ratio of farnesyl diphosphate to geranylgeranyl diphosphate increases to more than 2 under both conditions of substrate and product inhibition ?
geranyl diphosphate + 2 isopentenyl diphosphate
-
Methanothermobacter thermautotrophicus SF-4 geranylgeranyl diphosphate + 2 diphosphate
-
?
geranyl diphosphate + 2 isopentenyl diphosphate
-
Methanothermobacter thermautotrophicus SF-4 geranylgeranyl diphosphate + 2 diphosphate geranyl diphosphate + farnesyl diphosphate, ratio of farnesyl diphosphate to geranylgeranyl diphosphate increases to more than 2 under both conditions of substrate and product inhibition ?

Subunits

Subunits Comment Organism
dimer 2 * 39000, SDS-PAGE Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
assay at Methanothermobacter thermautotrophicus
65
-
-
Methanothermobacter thermautotrophicus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 72 approx. 30% of maximal activity at 30°C, approx. 80% of maximal activity at 72°C Methanothermobacter thermautotrophicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
in presence of 800 mM KCl, 30 min, stable Methanothermobacter thermautotrophicus
72
-
in the presence of 3500 mM KCl Methanothermobacter thermautotrophicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
-
Methanothermobacter thermautotrophicus