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Literature summary for 2.5.1.29 extracted from

  • Sagami, H.; Ishi, K.; Ogura, K.
    Geranylgeranylpyrophosphate synthetase of pig liver (1985), Methods Enzymol., 110, 184-188.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
diphosphate 10 mM, more than 90% inhibition Sus scrofa
farnesyl diphosphate above 0.025 mM Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates, less effective than Mg2+ Sus scrofa
Mg2+ required for activity Sus scrofa
Mg2+ less effective than Mn2+ Sus scrofa
Mn2+ required for activity Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
300000
-
gel filtration Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(E,E)-farnesyl diphosphate + isopentenyl diphosphate Sus scrofa no activity with dimethylallyl diphosphate geranylgeranyl diphosphate + diphosphate
-
?
geranyl diphosphate + 2 isopentenyl diphosphate Sus scrofa
-
geranylgeranyl diphosphate + 2 diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, DEAE Sephadex a-50, hydroxylapatite Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0000279
-
-
Sus scrofa

Storage Stability

Storage Stability Organism
-20°C, at least 1 month, no loss of activity Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(E,E)-farnesyl diphosphate + isopentenyl diphosphate no activity with dimethylallyl diphosphate Sus scrofa geranylgeranyl diphosphate + diphosphate
-
?
geranyl diphosphate + 2 isopentenyl diphosphate
-
Sus scrofa geranylgeranyl diphosphate + 2 diphosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Sus scrofa