Any feedback?
Literature summary for 2.5.1.29 extracted from
- Geranylgeranyl pyrophosphate synthetase lacking geranyl-transferring activity from Micrococcus luteus (1981), J. Biochem., 89, 1573-1580.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | stimulates farnesyl-transferring activity, inhibits dimethylallyl-transferring activity | Micrococcus luteus |  |
| Tween 80 | stimulates | Micrococcus luteus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diphosphate | 10 mM, complete inhibition | Micrococcus luteus | |
| Triton X-100 | stimulates farnesyl-transferring activity, inhibits dimethylallyl-transferring activity | Micrococcus luteus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
isopentenyl diphosphate | cosubstrate dimethylallyl diphosphate at 0.175 mM | Micrococcus luteus | |
| 0.062 | - |
dimethylallyl diphosphate | - |
Micrococcus luteus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for activity | Micrococcus luteus | |
| Mg2+ | optimal activation of farnesyl-transferring activity at 1 mM, optimal activation of dimethylallyl-transferring activity at 3 mM | Micrococcus luteus | |
| Mn2+ | slight activation | Micrococcus luteus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
gel filtration | Micrococcus luteus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (E,E)-farnesyl diphosphate + isopentenyl diphosphate | Micrococcus luteus | - |
geranylgeranyl diphosphate + diphosphate | - |
? | |
| geranyl diphosphate + 2 isopentenyl diphosphate | Micrococcus luteus | - |
geranylgeranyl diphosphate + 2 diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Micrococcus luteus | - |
the activities of EC 2.5.1.1 and EC 2.5.1.29 may be a mixture of 2 enzymes or a single enzyme with two independent catalytic sites, see EC 2.5.1.1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate, DEAE-Sephadex, hydroxylapatite, Sephadex G-100, enzyme fraction may be a mixture of EC 2.5.1.1 and EC 2.5.1.29 or a single enzyme with two independent catalytic sites, see EC 2.5.1.1 | Micrococcus luteus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0182 | - |
- |
Micrococcus luteus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (E)-3-methyl-2-dodecenyl diphosphate + isopentenyl diphosphate | - |
Micrococcus luteus | (2E,5E)-3,6-dimethylpentadeca-2,5-dien-1-yl diphosphate + diphosphate | - |
? | |
| (E,E)-farnesyl diphosphate + isopentenyl diphosphate | - |
Micrococcus luteus | geranylgeranyl diphosphate + diphosphate | - |
? | |
| dimethylallyl diphosphate + 3 isopentenyl diphosphate | - |
Micrococcus luteus | geranylgeranyl diphosphate + 3 diphosphate | - |
? | |
| geranyl diphosphate + 2 isopentenyl diphosphate | - |
Micrococcus luteus | geranylgeranyl diphosphate + 2 diphosphate | - |
? | |
| trans-3-methyl-2-octenyl diphosphate + isopentenyl diphosphate | similar activity as with dimethylallyl diphosphate | Micrococcus luteus | ? | - |
? | |
| trans-3-methyl-2-pentenyl diphosphate + isopentenyl diphosphate | 33% of activity with dimethylallyl diphosphate | Micrococcus luteus | ? | - |
? | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
