Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.27 extracted from

  • Chu, H.M.; Ko, T.P.; Wang, A.H.
    Crystal structure and substrate specificity of plant adenylate isopentenyltransferase from Humulus lupulus: distinctive binding affinity for purine and pyrimidine nucleotides (2010), Nucleic Acids Res., 38, 1738-1748.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.27

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed as a N-terminal hexahistidine tag protein in Escherichia coli BL21 (DE3) Humulus lupulus

Crystallization (Commentary)

Crystallization (Comment) Organism
The crystal structure of the AIPT-ATP complex from Humulus lupulus is similar to the previous structures of Agrobacterium AIPT and yeast tRNA-IPT. The enzyme is structurally homologous to the NTP-binding kinase family of proteins but forms a solvent-accessible channel that binds to the donor substrate dimethylallyl diphosphate, which is directed toward the acceptor substrate ATP/ADP. Humulus lupulus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dimethylallyl diphosphate + AMP Humulus lupulus
-
 diphosphate + N-(dimethylallyl)adenosine 5'-phosphate
-
 ir
dimethylallyl diphosphate + ATP Humulus lupulus
-
 N6-(dimethylallyl) adenosine 5'-triphosphate + diphosphate
-
 ir

Organism

Organism UniProt Comment Textmining
Humulus lupulus Q5GHF7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate + AMP
-
 Humulus lupulus diphosphate + N-(dimethylallyl)adenosine 5'-phosphate
-
 ir
dimethylallyl diphosphate + ATP
-
 Humulus lupulus N6-(dimethylallyl) adenosine 5'-triphosphate + diphosphate
-
 ir

Synonyms

Synonyms Comment Organism
adenylate isopentenyltransferase
-
 Humulus lupulus
AIPT
-
 Humulus lupulus
HlAIPT adenylate isopentenyltransferase-ATP complex from Humulus lupulus Humulus lupulus

General Information

General Information Comment Organism
metabolism initial reaction in the biosynthesis of cytokinins, which are important plant hormones Humulus lupulus