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Literature summary for 2.5.1.23 extracted from
- Biosynthesis of long-chain polyamines by crenarchaeal polyamine synthases from Hyperthermus butylicus and Pyrobaculum aerophilum (2009), FEBS Lett., 583, 3519-3524.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli with an N-terminal 6* His sequence | Hyperthermus butylicus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34437 | - |
x * 34437, calculation from sequence | Hyperthermus butylicus |
| 37000 | - |
x * 37000, SDS-PAGE | Hyperthermus butylicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hyperthermus butylicus | A2BJU2 | - |
- |
| Hyperthermus butylicus DSM 5456 | A2BJU2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Hyperthermus butylicus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.002 | - |
pH 7.5, 95°C, substrate: spermidine | Hyperthermus butylicus |
| 0.038 | - |
pH 7.5, 95°C, substrate: propane-1,3-diamine | Hyperthermus butylicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl 3-(methylthio)propylamine + propane-1,3-diamine | - |
Hyperthermus butylicus | S-methyl-5'-thioadenosine + bis(3-aminopropyl)amine | - |
? |  |
| S-adenosyl 3-(methylthio)propylamine + propane-1,3-diamine | - |
Hyperthermus butylicus DSM 5456 | S-methyl-5'-thioadenosine + bis(3-aminopropyl)amine | - |
? | |
| S-adenosyl 3-(methylthio)propylamine + spermidine | at 5% of the specific activity compared to propane-1,3-diamine | Hyperthermus butylicus | S-methyl-5'-thioadenosine + thermospermine + H+ | - |
? | |
| S-adenosyl 3-(methylthio)propylamine + spermidine | at 5% of the specific activity compared to propane-1,3-diamine | Hyperthermus butylicus DSM 5456 | S-methyl-5'-thioadenosine + thermospermine + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 37000, SDS-PAGE | Hyperthermus butylicus |
| ? | x * 34437, calculation from sequence | Hyperthermus butylicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HbSpe 1 | - |
Hyperthermus butylicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | - |
assay at | Hyperthermus butylicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Hyperthermus butylicus |
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Release 2023.1 (January 2023)
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