Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli XL-1 Blue cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
S165D/L175E/T178H/C206R | the mutant shows increased activity compared to the wild type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
S-adenosyl 3-(methylthio)propylamine | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens | |
0.0009 | - |
S-adenosyl 3-(methylthio)propylamine | mutant enzyme S165D/L175E/T178H/C206R, at pH 7.5 and 37°C | Homo sapiens | |
0.6 | - |
spermidine | mutant enzyme S165D/L175E/T178H/C206R, at pH 7.5 and 37°C | Homo sapiens | |
0.8 | - |
spermidine | wild type enzyme, at pH 7.5 and 37°C | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl 3-(methylthio)propylamine + spermidine | Homo sapiens | - |
S-methyl-5'-thioadenosine + spermine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
TALON affinity resin column chromatography | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
227 | - |
mutant enzyme S165D/L175E/T178H/C206R, at pH 7.5 and 37°C | Homo sapiens |
2425 | - |
wild type enzyme, at pH 7.5 and 37°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl 3-(methylthio)propylamine + spermidine | - |
Homo sapiens | S-methyl-5'-thioadenosine + spermine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SMS | - |
Homo sapiens |