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Literature summary for 2.5.1.21 extracted from

  • Gupta, N.; Sharma, P.; Santosh Kumar, R.J.; Vishwakarma, R.K.; Khan, B.M.
    Functional characterization and differential expression studies of squalene synthase from Withania somnifera (2012), Mol. Biol. Rep., 39, 8803-8812.
    View publication on PubMed
Search for more literature for 2.5.1.21

Cloned(Commentary)

Cloned (Comment) Organism
genes WsSQS1and WsSQS2, DNA and amino acid sequence determination and analysis, phylogenetic tree, quantitative real-time PCR tissue expression analysis, expression as soluble recombinant enzymes with 24 hydrophobic amino acids deleted from the C-terminus and expressed as His6-tagged proteins in Escherichia coli Withania somnifera

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, the catalytic site is composed of the large central cavity formed by antiparallel alpha helices with two aspartate rich regions (DXXXD) on opposite walls, these residues are considered to play roles in binding of prenyl phosphates by binding Mg2+ ions Withania somnifera

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 x * 43000, recombinant C-terminally truncated, His6-tagged WsSQS1and WsSQS2, SDS-PAGE Withania somnifera

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 (2E,6E)-farnesyl diphosphate Withania somnifera
-
 diphosphate + presqualene diphosphate
-
 ?
2 (2E,6E)-farnesyl diphosphate + NADPH + H+ Withania somnifera overall reaction squalene + 2 diphosphate + NADP+
-
 ?
presqualene diphosphate + NADPH + H+ Withania somnifera
-
 squalene + diphosphate + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Withania somnifera H9L9T8 WsSQS; genes WsSQS1and WsSQS2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged WsSQS1and WsSQS2 proteins from Escherichia coli by nickel affinity chromatgraphy Withania somnifera

Source Tissue

Source Tissue Comment Organism Textmining
leaf higher SQS comtent in younger than in mature leaves Withania somnifera
-
additional information quantitative real-time PCR tissue expression analysis, overview. WsSQS expresses more in young leaves than mature leaves, stem and root Withania somnifera
-
root
-
 Withania somnifera
-
stem
-
 Withania somnifera
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 (2E,6E)-farnesyl diphosphate
-
 Withania somnifera diphosphate + presqualene diphosphate
-
 ?
2 (2E,6E)-farnesyl diphosphate + NADPH + H+ overall reaction Withania somnifera squalene + 2 diphosphate + NADP+
-
 ?
presqualene diphosphate + NADPH + H+
-
 Withania somnifera squalene + diphosphate + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 43000, recombinant C-terminally truncated, His6-tagged WsSQS1and WsSQS2, SDS-PAGE Withania somnifera

Synonyms

Synonyms Comment Organism
SQS
-
 Withania somnifera

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Withania somnifera

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Withania somnifera

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Withania somnifera

General Information

General Information Comment Organism
metabolism squalene synthase is a key enzyme in the regulation of isoprenoid biosynthesis and is important in the withanolides biosynthesis pathway, overview Withania somnifera
additional information the catalytic site is composed of the large central cavity formed by antiparallel alpha helices with two aspartate rich regions (DXXXD) on opposite walls, these residues are considered to play roles in binding of prenyl phosphates by binding Mg2+ ions Withania somnifera