Cloned (Comment) | Organism |
---|---|
yeast enzyme overexpressed in Escherichia coli | Saccharomyces cerevisiae |
General Stability | Organism |
---|---|
a combination of 10% methanol, 10% glycerol, 30 mM octyl-beta-D-glucopyranoside, 0.4% Brij-58, and 1 mM DTT in 25 mM sodium phosphate, pH 7.4, essential for stability and maximal activity | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
farnesyl diphosphate | - |
Saccharomyces cerevisiae | |
0.5 | - |
NADPH | - |
Saccharomyces cerevisiae | |
3.6 | - |
NADH | - |
Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
farnesyl diphosphate + farnesyl diphosphate | Saccharomyces cerevisiae | essential enzyme in cholesterol biosynthetic pathway | diphosphate + presqualene diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
overexpressed in Escherichia coli | - |
Purification (Comment) | Organism |
---|---|
overexpressed in E. coli | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + NADH + H+ | - |
Saccharomyces cerevisiae | squalene + diphosphate + NAD+ | - |
? | |
farnesyl diphosphate + farnesyl diphosphate | - |
Saccharomyces cerevisiae | diphosphate + presqualene diphosphate | - |
? | |
farnesyl diphosphate + farnesyl diphosphate | essential enzyme in cholesterol biosynthetic pathway | Saccharomyces cerevisiae | diphosphate + presqualene diphosphate | - |
? | |
presqualene diphosphate + NAD(P)H | - |
Saccharomyces cerevisiae | squalene + NAD(P)+ + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000-44000, enzyme expressed in Escherichia coli, SDS-PAGE, carboxy-terminal truncated enzyme | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.53 | - |
farnesyl diphosphate | cosubstrate + NAD(P)H | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | preference for NADPH over NADH | Saccharomyces cerevisiae | |
NADPH | preference for NADPH over NADH | Saccharomyces cerevisiae |