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Literature summary for 2.5.1.21 extracted from

  • LoGrasso, P.V.; Soltis, D.A.; Boettcher, B.R.
    Overexpression, purification, and kinetic characterization of a carboxyl-terminal-truncated yeast squalene synthetase (1993), Arch. Biochem. Biophys., 307, 193-199.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
yeast enzyme overexpressed in Escherichia coli Saccharomyces cerevisiae

General Stability

General Stability Organism
a combination of 10% methanol, 10% glycerol, 30 mM octyl-beta-D-glucopyranoside, 0.4% Brij-58, and 1 mM DTT in 25 mM sodium phosphate, pH 7.4, essential for stability and maximal activity Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0025
-
farnesyl diphosphate
-
Saccharomyces cerevisiae
0.5
-
NADPH
-
Saccharomyces cerevisiae
3.6
-
NADH
-
Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
farnesyl diphosphate + farnesyl diphosphate Saccharomyces cerevisiae essential enzyme in cholesterol biosynthetic pathway diphosphate + presqualene diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
overexpressed in Escherichia coli
-

Purification (Commentary)

Purification (Comment) Organism
overexpressed in E. coli Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + NADH + H+
-
Saccharomyces cerevisiae squalene + diphosphate + NAD+
-
?
farnesyl diphosphate + farnesyl diphosphate
-
Saccharomyces cerevisiae diphosphate + presqualene diphosphate
-
?
farnesyl diphosphate + farnesyl diphosphate essential enzyme in cholesterol biosynthetic pathway Saccharomyces cerevisiae diphosphate + presqualene diphosphate
-
?
presqualene diphosphate + NAD(P)H
-
Saccharomyces cerevisiae squalene + NAD(P)+ + diphosphate
-
?

Subunits

Subunits Comment Organism
? x * 42000-44000, enzyme expressed in Escherichia coli, SDS-PAGE, carboxy-terminal truncated enzyme Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.53
-
farnesyl diphosphate cosubstrate + NAD(P)H Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NADH preference for NADPH over NADH Saccharomyces cerevisiae
NADPH preference for NADPH over NADH Saccharomyces cerevisiae