Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bovine serum albumin | activates recombinant enzyme | Saccharomyces cerevisiae | |
Tween 20 | stimulates recombinant enzyme | Saccharomyces cerevisiae | |
Tween 40 | stimulates recombinant enzyme | Saccharomyces cerevisiae | |
Tween 80 | stimulates recombinant enzyme | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
development of a plasmid for heterologous expression of yeast squalene synthase in Escherichia coli | Saccharomyces cerevisiae |
General Stability | Organism |
---|---|
methanol, 10%, does not stabilize | Saccharomyces cerevisiae |
octylglucoside, 5 mM, causes rapid loss of activity | Saccharomyces cerevisiae |
stable in buffer containing 5 mM octylglucoside and 15 mM CHAPS | Saccharomyces cerevisiae |
sucrose, 10%, does not stabilize | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-ethylmaleimide | - |
Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for squalene synthase reaction | Saccharomyces cerevisiae | |
Mg2+ | Mn2+ is more effective than Mg2+ in activation of dehydrosqualene formation | Saccharomyces cerevisiae | |
Mn2+ | stimulates, maximum stimulation at 0.1 mM | Saccharomyces cerevisiae | |
Mn2+ | more effective than Mg2+ in activation of dehydrosqualene formation | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
x * 44000, expressed in Escherichia coli, SDS-PAGE | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
farnesyl diphosphate + farnesyl diphosphate | Saccharomyces cerevisiae | first pathway-specific enzyme in cholesterol biosynthesis | diphosphate + presqualene diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
recombinant enzyme | - |
Purification (Comment) | Organism |
---|---|
expressed in E. coli | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-70°C, stable | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
farnesyl diphosphate + farnesyl diphosphate | - |
Saccharomyces cerevisiae | diphosphate + presqualene diphosphate | - |
? | |
farnesyl diphosphate + farnesyl diphosphate | first pathway-specific enzyme in cholesterol biosynthesis | Saccharomyces cerevisiae | diphosphate + presqualene diphosphate | - |
? | |
presqualene diphosphate + NAD(P)H | - |
Saccharomyces cerevisiae | squalene + NAD(P)+ + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 44000, expressed in Escherichia coli, SDS-PAGE | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.3 | - |
farnesyl diphosphate | in presence of 1% Tween 80 and Mg2+ | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.2 | 8.2 | pH 5.2: about 45% of maximum activity, pH 8.2: about 75% of maximum activity | Saccharomyces cerevisiae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
irreversible denaturation above | Saccharomyces cerevisiae |