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Literature summary for 2.5.1.21 extracted from
- Formation of dehydrosqualene catalyzed by squalene synthetase in Saccharomyces cerevisiae (1982), J. Biochem., 91, 911-921.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Sus scrofa | - |
- |
| microsome | - |
Saccharomyces cerevisiae | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | no effect | Sus scrofa |  |
| Mg2+ | Mn2+ is six times more effective in activation than Mg2+ | Saccharomyces cerevisiae | |
| Mn2+ | stimulation | Sus scrofa | |
| Mn2+ | six times more effective in activation than Mg2+ | Saccharomyces cerevisiae | |
| Mn2+ | in presence of Mn2+ and Mg2+ the activity is reduced to one-third of that in presence of Mn2+ alone | Sus scrofa | |
| Mn2+ | in presence of Mn2+ and Mg2+ the activity is reduced to one-third of that in presence of Mn2+ alone | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 68000 | - |
gel filtration | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Saccharomyces cerevisiae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| farnesyl diphosphate | - |
Sus scrofa | 12,13-cis-dehydrosqualene + diphosphate | - |
? | |
| farnesyl diphosphate | - |
Saccharomyces cerevisiae | 12,13-cis-dehydrosqualene + diphosphate | - |
? | |
| farnesyl diphosphate + NAD(P)H | - |
Sus scrofa | squalene + diphosphate + NAD(P)+ | - |
? | |
| farnesyl diphosphate + NAD(P)H | - |
Saccharomyces cerevisiae | squalene + diphosphate + NAD(P)+ | - |
? | |
| presqualene diphosphate + NAD(P)H | in presence of reducing pyridine nucleotide, preferably NADPH, squalene is formed, in absence of reducing cofactor the rate of the condensation reaction is lower and all of the product accumulates as presqualene diphosphate | Sus scrofa | squalene + NAD(P)+ + diphosphate | - |
? | |
| presqualene diphosphate + NAD(P)H | in presence of reducing pyridine nucleotide, preferably NADPH, squalene is formed, in absence of reducing cofactor the rate of the condensation reaction is lower and all of the product accumulates as presqualene diphosphate | Saccharomyces cerevisiae | squalene + NAD(P)+ + diphosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Sus scrofa |
| 30 | - |
assay at | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Sus scrofa | |
| NADPH | - |
Saccharomyces cerevisiae | |
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Release 2023.1 (January 2023)
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