Application | Comment | Organism |
---|---|---|
drug development | protonated enolpyruvylshikimate 3-phosphate (cation) intermediate as potential target for inhibitor design | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate | enolpyruvylshikimate 3-phosphate ketal is catalytically formed as side product upon enolpyruvyl activation through protonation by an intramolecular nucleophilic addition of O4 to C2 of the enolpyruvyl moiety | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-enolpyruvylshikimate 3-phosphate + H2O | enzymatic hydrolysis, pH 7.0, 25°C, in presence of phosphate scavenging system and excess of enzyme | Escherichia coli | 5-enolpyruvylshikimate 3-phosphate ketal | - |
? | |
phosphoenolpyruvate + 3-phosphoshikimate | the enzyme must promote breakdown of enolpyruvylshikimate 3-phosphate though enolpyruvyl activation, forming either the enolpyruvylshikimate 3-phosphate cation or a highly cation-like transition state | Escherichia coli | phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate | - |
? | |
shikimate 3-phosphate + phosphoenolpyruvate | enolpyruvylshikimate 3-phosphate ketal formation from shikimate 3-phosphate (radiolabelled) and phosphoenolypyruvate (in excess over shikimate 3-phosphate) by hydrolysis of product 5-enpolpyruvylshikimate 3-phosphate in presence of 25% excess of enzyme, pH 7.0, 25°C | Escherichia coli | phosphate + 5-enpolpyruvylshikimate 3-phosphate | analysis of products by ion-paired C18 reverse-phase HPLC and scintillation counting | ? |
Synonyms | Comment | Organism |
---|---|---|
AroA | - |
Escherichia coli |
enolpyruvylshikimate 3-phosphate synthase | - |
Escherichia coli |
enolpyruvylshikimate-3-phosphate synthase | - |
Escherichia coli |
EPSP synthase | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000045 | - |
phosphoenolpyruvate | 5-enpolpyruvylshikimate 3-phosphate ketal formation in presence of shikimate 3-phosphate and equimolar phosphoenolpyruvate | Escherichia coli | |
0.000012 | - |
5-enolpyruvylshikimate 3-phosphate | 5-enpolpyruvylshikimate 3-phosphate ketal formation by hydrolysis of radiolabelled 5-enpolpyruvylshikimate 3-phosphate, pH 7.0, 25°C | Escherichia coli | |
0.000049 | - |
phosphoenolpyruvate | 5-enpolpyruvylshikimate 3-phosphate ketal formation in presence of shikimate 3-phosphate and 6.7 equivalents of phosphoenolpyruvate | Escherichia coli | |
0.00008 | - |
phosphoenolpyruvate | 5-enpolpyruvylshikimate 3-phosphate ketal formation in presence of shikimate 3-phosphate and 6.7 equivalents of phosphoenolpyruvate and 10 mM phosphate | Escherichia coli |