Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-phosphonomethylglycine | 0.0183 mM, 50% inhibition, 0.5 mM 96% inhibition, competitive vs. phosphoenolpyruvate, uncompetitive vs. shikimate 3-phosphate at pH 7.0, mechanism; pH-dependent | Nicotiana sylvestris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana sylvestris | - |
cv. Speg et Comes, aneuploid cell line ANS-1 | - |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose, CM-cellulose, partial purification | Nicotiana sylvestris |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Nicotiana sylvestris | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
16.9 | - |
- |
Nicotiana sylvestris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + 3-phosphoshikimate | - |
Nicotiana sylvestris | phosphate + 5-enolpyruvylshikimate 3-phosphate | i.e. 5-O-(1-carboxyvinyl)-3-phosphoshikimate | r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.2 | 50% of maximal activity between pH 6.2 and pH 8.5 | Nicotiana sylvestris |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.2 | 8.5 | approx. half-maximal activity at pH 6.2 and 8.5 | Nicotiana sylvestris |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00125 | - |
N-phosphonomethylglycine | vs. phosphoenolpyruvate | Nicotiana sylvestris | |
0.0183 | - |
N-phosphonomethylglycine | vs. shikimate 3-phosphate | Nicotiana sylvestris |