KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten steady-state kinetics | Phaseolus vulgaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phaseolus vulgaris | - |
isozymes PvGSTF1-1, PvGSTU2-2 and PvGSTU3-3 | - |
Purification (Comment) | Organism |
---|---|
native fluazifop-p-butyl-inducible GSTs PvGSTF1-1, PvGSTU2-2 and PvGSTU3-3 from leaves by glutathione affinity chromatography and dialysis | Phaseolus vulgaris |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
RX + glutathione = HX + R-S-glutathione | substrate specificity and catalytic mechanism, overview | Phaseolus vulgaris |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Phaseolus vulgaris | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2,3-dichloro-4-[2-methylenebutyryl]phenoxy)acetic acid + glutathione | i.e. ethacrynic acid | Phaseolus vulgaris | ? | - |
? | |
1-bromo-2,4-dinitrobenzene + glutathione | - |
Phaseolus vulgaris | S-(2,4-dinitrophenyl)glutathione + HBr | - |
? | |
1-chloro-2,4-dinitrobenzene + glutathione | - |
Phaseolus vulgaris | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? | |
1-fluoro-2,4-dinitrobenzene + glutathione | - |
Phaseolus vulgaris | S-(2,4-dinitrophenyl)glutathione + HF | - |
? | |
1-iodo-2,4-dinitrobenzene + glutathione | - |
Phaseolus vulgaris | S-(2,4-dinitrophenyl)glutathione + HI | - |
? | |
2-hydroxyethyl disulfide + glutathione | - |
Phaseolus vulgaris | ? | - |
? | |
4-chloro-7-nitrobenzofurazan + glutathione | - |
Phaseolus vulgaris | ? | - |
? | |
4-nitrobenzyl chloride + glutathione | - |
Phaseolus vulgaris | S-(4-nitrobenzyl)glutathione + HCl | - |
? | |
alachlor + glutathione | - |
Phaseolus vulgaris | ? | - |
? | |
allyl isothiocyanate + glutathione | - |
Phaseolus vulgaris | ? | - |
? | |
atrazine + glutathione | - |
Phaseolus vulgaris | ? | - |
? | |
bromosulfophthalein + glutathione | - |
Phaseolus vulgaris | ? | - |
? | |
dehydroascorbate + glutathione | - |
Phaseolus vulgaris | ? | - |
? | |
fluazifop-p-butyl + glutathione | - |
Phaseolus vulgaris | ? | - |
? | |
fluorodifen + glutathione | - |
Phaseolus vulgaris | ? | - |
? | |
metolachlor + glutathione | - |
Phaseolus vulgaris | ? | - |
? | |
additional information | fluazifop-inducible glutathione transferase isozymes catalyze a broad range of reactions and exhibit quite varied substrate specificity, molecular modeling and structural analysis reveal key structural characteristics, isozynme substrate specificities and catalytic mechanism, overview. Only PvGSTU2-2 shows appreciable activity towards trans-2-nonenal, but the isozymes are also active with cumene hydroperoxide, tert-butyl hydroperoxide, and lauroyl peroxide, no activity of all isozymes with benzoyl peroxide | Phaseolus vulgaris | ? | - |
? | |
phenethyl isothiocyanate + glutathione | - |
Phaseolus vulgaris | ? | - |
? | |
trans-2-nonenal + glutathione | - |
Phaseolus vulgaris | ? | - |
? | |
trans-4-phenyl-3-buten-2-one + glutathione | - |
Phaseolus vulgaris | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GST | - |
Phaseolus vulgaris |
PvGSTF1-1 | - |
Phaseolus vulgaris |
PvGSTU2-2 | - |
Phaseolus vulgaris |
PvGSTU3-3 | - |
Phaseolus vulgaris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Phaseolus vulgaris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Phaseolus vulgaris |
Organism | Comment | Expression |
---|---|---|
Phaseolus vulgaris | treatment of Phaseolus vulgaris leaves with the aryloxyphenoxypropionic herbicide fluazifop-p-butyl, i.e. butyl 2-[4-[[5-(trifluoromethyl)-2-pyridinyl]oxy]phenoxy]propanoate, results in induction of three GST isozyme activities, i.e. PvGSTF1-1, PvGSTU2-2 and PvGSTU3-3 | up |