Cloned (Comment) | Organism |
---|---|
expression of N-terminally Strep-tagged AtGSTF2 and AtGSTF3 in Escherichia coli, expression of untagged GSTF2 | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | isothermal titration calorimetry reveals high-affinity binding for GSTF2 and GSTF3, which is enhanced in the presence of glutathione and by the other heterocyclic ligands, and allosteric enhancement in glutathione-conjugating activity | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | GSTF2 association with membrane vesicles | Arabidopsis thaliana | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally Strep-tagged AtGSTF2 and AtGSTF3 from Escherichia coli by affinity chromatography and gel filtration | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | plant-specific phi class of glutathione transferases, GSTFs, are often highly stress-inducible and expressed in a tissue-specific manner | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | overexpressed in bacteria, family members GSTF2 and GSTF3 bind a series of heterocyclic compounds, including lumichrome, harmane, norharmane and indole-3-aldehyde. GSTF2 also selectively binds the indole-derived phytoalexin camalexin, as well as the flavonol quercetin-3-O-rhamnoside, recombinant GSTF2 ligand binding studies and kinetics, overview | Arabidopsis thaliana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AtGSTF2 | - |
Arabidopsis thaliana |
phi class glutathione transferase | - |
Arabidopsis thaliana |
Organism | Comment | Expression |
---|---|---|
Arabidopsis thaliana | plant-specific phi class of glutathione transferases, GSTFs, are often highly stress-inducible and expressed in a tissue-specific manner | up |
General Information | Comment | Organism |
---|---|---|
physiological function | GSTF2 and GSTF3 may have roles in regulating the binding and transport of defence-related compounds in planta | Arabidopsis thaliana |