Literature summary for 2.5.1.18 extracted from

Achilonu, I.; Gildenhuys, S.; Fisher, L.; Burke, J.; Fanucchi, S.; Sewell, B.T.; Fernandes, M.; Dirr, H.W.
The role of a topologically conserved isoleucine in glutathione transferase structure, stability and function (2010), Acta Crystallogr. Sect. F, 66, 776-780.

Cloned(Commentary)

Cloned (Comment)	Organism
expression of wild-type GSTA1-1 and mutants in Escherichia coli strain BL21 (DE3) pLysS	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
recombinant I71A and I71V hGSTA1-1, hanging drop vapour diffusion method, protein solution, containing 10 mg/ml I71A hGSTA1-1 or 15 mg/ml I71V hGSTA1-1 in 0.1 M Tris-HCl, pH 7.5, 10 mM DTT, 2.5 mM S-hexylglutathione and 0.02% sodium azide is mixed with an equal volume of reservoir solution containing 19%w/v PEG 4000, 0.1 M Tris-HCl, pH 7.5, 10 mM DTT, and 0.02% sodium azide, 20°C, X-ray diffraction structure determmination and analysis at 1.75-2.51 A resolution	Homo sapiens

Crystallization (Comment)

Organism

recombinant I71A and I71V hGSTA1-1, hanging drop vapour diffusion method, protein solution, containing 10 mg/ml I71A hGSTA1-1 or 15 mg/ml I71V hGSTA1-1 in 0.1 M Tris-HCl, pH 7.5, 10 mM DTT, 2.5 mM S-hexylglutathione and 0.02% sodium azide is mixed with an equal volume of reservoir solution containing 19%w/v PEG 4000, 0.1 M Tris-HCl, pH 7.5, 10 mM DTT, and 0.02% sodium azide, 20°C, X-ray diffraction structure determmination and analysis at 1.75-2.51 A resolution

Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
I71V	site-directed mutagensis, structure analysis	Homo sapiens
II71A	site-directed mutagensis, structure analysis	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P08263	isozyme GSTA1-1	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1-chloro-2,4-dinitrobenzene + glutathione	-	Homo sapiens	S-(2,4-dinitrophenyl)glutathione + HCl	-	?

Synonyms

Synonyms	Comment	Organism
glutathione-transferase	-	Homo sapiens
GST	-	Homo sapiens
GSTA1-1	-	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	assay at	Homo sapiens

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Release 2023.1 (January 2023)