Cloned (Comment) | Organism |
---|---|
expression of wild-type GSTA1-1 and mutants in Escherichia coli strain BL21 (DE3) pLysS | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
recombinant I71A and I71V hGSTA1-1, hanging drop vapour diffusion method, protein solution, containing 10 mg/ml I71A hGSTA1-1 or 15 mg/ml I71V hGSTA1-1 in 0.1 M Tris-HCl, pH 7.5, 10 mM DTT, 2.5 mM S-hexylglutathione and 0.02% sodium azide is mixed with an equal volume of reservoir solution containing 19%w/v PEG 4000, 0.1 M Tris-HCl, pH 7.5, 10 mM DTT, and 0.02% sodium azide, 20°C, X-ray diffraction structure determmination and analysis at 1.75-2.51 A resolution | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
I71V | site-directed mutagensis, structure analysis | Homo sapiens |
II71A | site-directed mutagensis, structure analysis | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P08263 | isozyme GSTA1-1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-chloro-2,4-dinitrobenzene + glutathione | - |
Homo sapiens | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glutathione-transferase | - |
Homo sapiens |
GST | - |
Homo sapiens |
GSTA1-1 | - |
Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Homo sapiens |