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Literature summary for 2.5.1.18 extracted from
- The role of the Aedes aegypti Epsilon glutathione transferases in conferring resistance to DDT and pyrethroid insecticides (2011), Insect Biochem. Mol. Biol., 41, 203-209.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the Epsilon GST class in Aedes aegypti consists of eight sequentially arranged genes spanning 53645 bp on super contig 1.291, which maps to chromosome 2, quantitative RT-PCR isozyme GSTE1-GSTE8 expression analysis in different strains, overview. Overexpression of N-terminally His6-tagged Epsilon GST isozymmes GSTe1, GSTe2, GSTe8, GSTe5, and GSTe7, in Escherichia coli strain BL21 Star (DE3) | Aedes aegypti |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
1-chloro-2,4-dinitrobenzene | recombinant His6-tagged isozyme GSTE2-2 from strain PMD-R, pH and temperature not specified in the publication | Aedes aegypti |  |
| 0.05 | - |
1-chloro-2,4-dinitrobenzene | recombinant His6-tagged isozyme GSTE2-2 from strain NO, pH and temperature not specified in the publication | Aedes aegypti | |
| 0.08 | - |
1-chloro-2,4-dinitrobenzene | recombinant His6-tagged isozyme GSTE4-4, pH and temperature not specified in the publication | Aedes aegypti | |
| 0.19 | - |
1-chloro-2,4-dinitrobenzene | recombinant His6-tagged isozyme GSTE5-5, pH and temperature not specified in the publication | Aedes aegypti | |
| 0.35 | - |
1-chloro-2,4-dinitrobenzene | recombinant His6-tagged isozyme GSTE8-8, pH and temperature not specified in the publication | Aedes aegypti | |
| 0.41 | - |
1-chloro-2,4-dinitrobenzene | recombinant His6-tagged isozyme GSTE3-3, pH and temperature not specified in the publication | Aedes aegypti | |
| 0.46 | - |
1-chloro-2,4-dinitrobenzene | recombinant His6-tagged isozyme GSTE7-7, pH and temperature not specified in the publication | Aedes aegypti | |
| 2.2 | - |
glutathione | recombinant His6-tagged isozyme GSTE7-7, pH and temperature not specified in the publication | Aedes aegypti | |
| 3 | - |
glutathione | recombinant His6-tagged isozyme GSTE3-3, pH and temperature not specified in the publication | Aedes aegypti | |
| 3.8 | - |
glutathione | recombinant His6-tagged isozyme GSTE4-4, pH and temperature not specified in the publication | Aedes aegypti | |
| 4.1 | - |
glutathione | recombinant His6-tagged isozyme GSTE8-8, pH and temperature not specified in the publication | Aedes aegypti | |
| 7 | - |
glutathione | recombinant His6-tagged isozyme GSTE2-2 from strain PMD-R, pH and temperature not specified in the publication | Aedes aegypti | |
| 13.2 | - |
glutathione | recombinant His6-tagged isozyme GSTE5-5, pH and temperature not specified in the publication | Aedes aegypti | |
| 13.6 | - |
glutathione | recombinant His6-tagged isozyme GSTE2-2 from strain NO, pH and temperature not specified in the publication | Aedes aegypti | |
| 26.1 | - |
glutathione | recombinant His6-tagged isozyme GSTE1-1, pH and temperature not specified in the publication | Aedes aegypti | |
| 113 | - |
1-chloro-2,4-dinitrobenzene | recombinant His6-tagged isozyme GSTE1-1, pH and temperature not specified in the publication | Aedes aegypti | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Aedes aegypti | only isozymes GSTE2-2 and GSTE5-5 are able to metabolize DDT to DDE | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aedes aegypti | - |
PMD and PMD-R strains, the latter is a highly resistant strain, Isla Mujeres (IM) strain, New Orleans (NO) susceptible strain, isozymes GSTE1-GSTE8 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant N-terminally His6-tagged Epsilon GSTs from Escherichia cli by nickel affinity chromatography and gel filtration | Aedes aegypti |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| larva | - |
Aedes aegypti | - |
| additional information | developmental stage- and gender-specific expression of isozymes GSTE1-GSTE8, overview | Aedes aegypti | - |
| pupa | - |
Aedes aegypti | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
of the seven Epsilon GSTs GSTE1-GSTE5, GSTE7 and GSTE8, GSTE3-3 has the highest maximum velocity of 61.5 micromol/min/mg protein with highest turnover rates being achieved by GSTE3-3 and GSTE5-5 | Aedes aegypti |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-chloro-2,4-dinitrobenzene + glutathione | - |
Aedes aegypti | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? | |
| additional information | only isozymes GSTE2-2 and GSTE5-5 are able to metabolize DDT to DDE | Aedes aegypti | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Epsilon glutathione transferase | - |
Aedes aegypti |
| Epsilon GST | - |
Aedes aegypti |
| GSTE1 | - |
Aedes aegypti |
| GSTE2 | - |
Aedes aegypti |
| GSTE3 | - |
Aedes aegypti |
| GSTE4 | - |
Aedes aegypti |
| GSTE5 | - |
Aedes aegypti |
| GSTE6 | - |
Aedes aegypti |
| GSTE7 | - |
Aedes aegypti |
| GSTE8 | - |
Aedes aegypti |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6 | - |
glutathione | recombinant His6-tagged isozyme GSTE8-8, pH and temperature not specified in the publication | Aedes aegypti | |
| 6 | - |
1-chloro-2,4-dinitrobenzene | recombinant His6-tagged isozyme GSTE8-8, pH and temperature not specified in the publication | Aedes aegypti | |
| 7 | - |
glutathione | recombinant His6-tagged isozyme GSTE7-7, pH and temperature not specified in the publication | Aedes aegypti | |
| 7 | - |
1-chloro-2,4-dinitrobenzene | recombinant His6-tagged isozyme GSTE7-7, pH and temperature not specified in the publication | Aedes aegypti | |
| 10 | - |
glutathione | recombinant His6-tagged isozyme GSTE1-1, pH and temperature not specified in the publication | Aedes aegypti | |
| 10 | - |
1-chloro-2,4-dinitrobenzene | recombinant His6-tagged isozyme GSTE1-1, pH and temperature not specified in the publication | Aedes aegypti | |
| 11 | - |
glutathione | recombinant His6-tagged isozyme GSTE2-2 from strain NO, pH and temperature not specified in the publication | Aedes aegypti | |
| 11 | - |
1-chloro-2,4-dinitrobenzene | recombinant His6-tagged isozyme GSTE2-2 from strain NO, pH and temperature not specified in the publication | Aedes aegypti | |
| 23 | - |
glutathione | recombinant His6-tagged isozyme GSTE2-2 from strain PMD-R, pH and temperature not specified in the publication | Aedes aegypti | |
| 23 | - |
1-chloro-2,4-dinitrobenzene | recombinant His6-tagged isozyme GSTE2-2 from strain PMD-R, pH and temperature not specified in the publication | Aedes aegypti | |
| 23 | - |
glutathione | recombinant His6-tagged isozyme GSTE4-4, pH and temperature not specified in the publication | Aedes aegypti | |
| 23 | - |
1-chloro-2,4-dinitrobenzene | recombinant His6-tagged isozyme GSTE4-4, pH and temperature not specified in the publication | Aedes aegypti | |
| 46 | - |
glutathione | recombinant His6-tagged isozyme GSTE3-3, pH and temperature not specified in the publication | Aedes aegypti | |
| 46 | - |
1-chloro-2,4-dinitrobenzene | recombinant His6-tagged isozyme GSTE3-3, pH and temperature not specified in the publication | Aedes aegypti | |
| 51 | - |
glutathione | recombinant His6-tagged isozyme GSTE5-5, pH and temperature not specified in the publication | Aedes aegypti | |
| 51 | - |
1-chloro-2,4-dinitrobenzene | recombinant His6-tagged isozyme GSTE5-5, pH and temperature not specified in the publication | Aedes aegypti | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | partial silencing of either GSTe7 or GSTe2 by RNA interference results in an increased susceptibility to the pyrethroid, deltamethrin | Aedes aegypti |
| physiological function | GSTE2 is implicated in conferring resistance to DDT for the dengue vector Aedes aegypti. Epsilon GSTs have DDT dehydrochlorinase activity, GSTe2 and two additional Epsilon GST genes, GSTe5 and GSTe7, are expressed at elevated levels in the resistant population and the recombinant homodimer GSTE5-5 also exhibits low levels of DDT dehydrochlorinase activity | Aedes aegypti |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
glutathione | recombinant His6-tagged isozyme GSTE1-1, pH and temperature not specified in the publication | Aedes aegypti | |
| 0.8 | - |
glutathione | recombinant His6-tagged isozyme GSTE2-2 from strain NO, pH and temperature not specified in the publication | Aedes aegypti | |
| 1.4 | - |
glutathione | recombinant His6-tagged isozyme GSTE8-8, pH and temperature not specified in the publication | Aedes aegypti | |
| 3.2 | - |
glutathione | recombinant His6-tagged isozyme GSTE7-7, pH and temperature not specified in the publication | Aedes aegypti | |
| 3.3 | - |
glutathione | recombinant His6-tagged isozyme GSTE2-2 from strain PMD-R, pH and temperature not specified in the publication | Aedes aegypti | |
| 3.9 | - |
glutathione | recombinant His6-tagged isozyme GSTE5-5, pH and temperature not specified in the publication | Aedes aegypti | |
| 5.9 | - |
glutathione | recombinant His6-tagged isozyme GSTE4-4, pH and temperature not specified in the publication | Aedes aegypti | |
| 9 | - |
1-chloro-2,4-dinitrobenzene | recombinant His6-tagged isozyme GSTE1-1, pH and temperature not specified in the publication | Aedes aegypti | |
| 15 | - |
glutathione | recombinant His6-tagged isozyme GSTE3-3, pH and temperature not specified in the publication | Aedes aegypti | |
| 15 | - |
1-chloro-2,4-dinitrobenzene | recombinant His6-tagged isozyme GSTE7-7, pH and temperature not specified in the publication | Aedes aegypti | |
| 17 | - |
1-chloro-2,4-dinitrobenzene | recombinant His6-tagged isozyme GSTE8-8, pH and temperature not specified in the publication | Aedes aegypti | |
| 111 | - |
1-chloro-2,4-dinitrobenzene | recombinant His6-tagged isozyme GSTE3-3, pH and temperature not specified in the publication | Aedes aegypti | |
| 220 | - |
1-chloro-2,4-dinitrobenzene | recombinant His6-tagged isozyme GSTE2-2 from strain NO, pH and temperature not specified in the publication | Aedes aegypti | |
| 268 | - |
1-chloro-2,4-dinitrobenzene | recombinant His6-tagged isozyme GSTE5-5, pH and temperature not specified in the publication | Aedes aegypti | |
| 282 | - |
1-chloro-2,4-dinitrobenzene | recombinant His6-tagged isozyme GSTE4-4, pH and temperature not specified in the publication | Aedes aegypti | |
| 763 | - |
1-chloro-2,4-dinitrobenzene | recombinant His6-tagged isozyme GSTE2-2 from strain PMD-R, pH and temperature not specified in the publication | Aedes aegypti | |
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