Application | Comment | Organism |
---|---|---|
drug development | glutathione S-transferases of Plasmodium parasites are potential targets for antimalarial drug and vaccine development | Plasmodium vivax |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme immobilization by glutaraldehyde cross-linking, overview | Plasmodium vivax |
General Stability | Organism |
---|---|
unfolding behavior of Plasmodium vivax GST is significantly different from Plasmodium falciparum GST, the unfolding pathway of Plasmodium vivax GST is non-cooperative with stabilization of an inactive dimeric intermediate | Plasmodium vivax |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | presence of salts effectively inhibits PvGST enzymatic activity by quenching the nucleophilicity of the thiolate anion of GSH, relative decrease in descending order MgCl2, MgSO4, NaCl, Na2SO4 | Plasmodium vivax |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
x * 25000, recombinant enzyme, SDS-PAGE | Plasmodium vivax |
50000 | - |
recombinant dimeric enzyme, gel filtration | Plasmodium vivax |
100000 | - |
recombinant tetrameric enzyme, gel filtration | Plasmodium vivax |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Plasmodium vivax | the regulation of GST enzymatic activity through a dimer-tetramer transition via GSH binding is an exclusive feature of Plasmodium, three-dimensional modeling, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium vivax | Q0ZS46 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + 1-chloro-2,4-dinitrobenzene | - |
Plasmodium vivax | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? | |
additional information | the regulation of GST enzymatic activity through a dimer-tetramer transition via GSH binding is an exclusive feature of Plasmodium, three-dimensional modeling, overview | Plasmodium vivax | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer or tetramer | x * 25000, recombinant enzyme, SDS-PAGE | Plasmodium vivax |
More | unfolding behavior of Plasmodium vivax GST is significantly different from Plasmodium falciparum GST, the unfolding pathway of Plasmodium vivax GST is non-cooperative with stabilization of an inactive dimeric intermediate. The absence of any compact folded monomeric intermediate during the unfolding transition suggests that inter-subunit interactions play an important role in stabilizing the protein, overview. Three-dimensional modeling, overview | Plasmodium vivax |
Synonyms | Comment | Organism |
---|---|---|
glutathione S-transferase | - |
Plasmodium vivax |
GST | - |
Plasmodium vivax |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Plasmodium vivax |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Plasmodium vivax |