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Literature summary for 2.5.1.18 extracted from

  • Feil, S.C.; Tang, J.; Hansen, G.; Gorman, M.A.; Wiktelius, E.; Stenberg, G.; Parker, M.W.
    Crystallization and preliminary X-ray analysis of glutathione transferases from cyanobacteria (2009), Acta Crystallogr. Sect. F, 65, 475-477.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermosynechococcus vestitus
expression in Escherichia coli Synechococcus elongatus

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme, hanging-drop vapour-diffusion method, 22°C, 0.002 ml of 6 mg/ml protein in 10 mM Tris-HCl buffer, pH 7.8, and 1 mM EDTA, is mixed with 0.002 ml of reservoir solution containing 25-35% v/v pentaerythritol propoxylate, 0.2 M potassium chloride and 50 mM HEPES buffer, pH 7.5, 3 days, X-ray diffraction structure determination and analysis at 1.45 A resolution, molecular replacement Thermosynechococcus vestitus
purified recombinant enzyme, sitting-drop vapour-diffusion method, 100 nl of 3 mg/ml protein solution is mixed with 100 nl of reservoir solution containing 20% w/v PEG 6000, 0.2 M CaCl2 and 100 mM HEPES buffer, pH 7.5 or 100 mM MES buffer, pH 6.5, 5 days, second variant by hanging-drop vapour-diffusion method, 0.002 ml protein solution are mixed with an equal volume of reservoir solution containing 13-18% w/v PEG 6000, 0.2 M CaCl2 and 100 mM MES buffer, pH 6.5, equilibration against 1 ml reservoir solution, 3 weeks, X-ray diffraction structure determination and analysis at 2.0 A resolution, molecular replacement Synechococcus elongatus

Organism

Organism UniProt Comment Textmining
Synechococcus elongatus
-
PCC 6301
-
Thermosynechococcus vestitus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli by glutathione affinity chromatography and dialysis Thermosynechococcus vestitus
recombinant enzyme from Escherichia coli by glutathione affinity chromatography and dialysis Synechococcus elongatus

Synonyms

Synonyms Comment Organism
Chi GST
-
Thermosynechococcus vestitus
Chi GST
-
Synechococcus elongatus
glutathione S-transferase
-
Thermosynechococcus vestitus
glutathione S-transferase
-
Synechococcus elongatus
GST
-
Thermosynechococcus vestitus
GST
-
Synechococcus elongatus