Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermosynechococcus vestitus |
expression in Escherichia coli | Synechococcus elongatus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, hanging-drop vapour-diffusion method, 22°C, 0.002 ml of 6 mg/ml protein in 10 mM Tris-HCl buffer, pH 7.8, and 1 mM EDTA, is mixed with 0.002 ml of reservoir solution containing 25-35% v/v pentaerythritol propoxylate, 0.2 M potassium chloride and 50 mM HEPES buffer, pH 7.5, 3 days, X-ray diffraction structure determination and analysis at 1.45 A resolution, molecular replacement | Thermosynechococcus vestitus |
purified recombinant enzyme, sitting-drop vapour-diffusion method, 100 nl of 3 mg/ml protein solution is mixed with 100 nl of reservoir solution containing 20% w/v PEG 6000, 0.2 M CaCl2 and 100 mM HEPES buffer, pH 7.5 or 100 mM MES buffer, pH 6.5, 5 days, second variant by hanging-drop vapour-diffusion method, 0.002 ml protein solution are mixed with an equal volume of reservoir solution containing 13-18% w/v PEG 6000, 0.2 M CaCl2 and 100 mM MES buffer, pH 6.5, equilibration against 1 ml reservoir solution, 3 weeks, X-ray diffraction structure determination and analysis at 2.0 A resolution, molecular replacement | Synechococcus elongatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechococcus elongatus | - |
PCC 6301 | - |
Thermosynechococcus vestitus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli by glutathione affinity chromatography and dialysis | Thermosynechococcus vestitus |
recombinant enzyme from Escherichia coli by glutathione affinity chromatography and dialysis | Synechococcus elongatus |
Synonyms | Comment | Organism |
---|---|---|
Chi GST | - |
Thermosynechococcus vestitus |
Chi GST | - |
Synechococcus elongatus |
glutathione S-transferase | - |
Thermosynechococcus vestitus |
glutathione S-transferase | - |
Synechococcus elongatus |
GST | - |
Thermosynechococcus vestitus |
GST | - |
Synechococcus elongatus |