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Literature summary for 2.5.1.18 extracted from

  • Zhang, Z.R.; Bai, M.; Wang, X.Y.; Zhou, J.M.; Perrett, S.
    Restoration of glutathione transferase activity by single-site mutation of the yeast prion protein Ure2 (2008), J. Mol. Biol., 384, 641-651.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol 1 mM dithiothreitol stimulates activity of the Ure2p mutant A122C Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
A122C the single-site mutation A122C and N124A/V (but not N124S/Y/C) restores the GST activity of Ure2p protein toward 1-chloro-2,4-dinitrobenzene, while causing a substantial reduction in glutathione peroxidase activity Saccharomyces cerevisiae
N124V the single-site mutation A122C and N124A/V (but not N124S/Y/C) restores the GST activity of Ure2p protein toward 1-chloro-2,4-dinitrobenzene, while causing a substantial reduction in glutathione peroxidase activity Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
glutathione Ure2p mutant A122C, at 25°C and pH 8.0, in the presence of 1 mM dithiothreitol Saccharomyces cerevisiae
5.8
-
glutathione Ure2p mutant N124V, at 25°C and pH 8.0 Saccharomyces cerevisiae
7.2
-
1-chloro-2,4-dinitrobenzene Ure2p mutant A122C, at 25°C and pH 8.0, in the presence of 1 mM dithiothreitol Saccharomyces cerevisiae
29.2
-
1-chloro-2,4-dinitrobenzene Ure2p mutant N124V, at 25°C and pH 8.0 Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
the single-site mutation A122C and N124A/V (but not N124S/Y/C) restores the GST activity of Ure2p protein toward 1-chloro-2,4-dinitrobenzene, while causing a substantial reduction in glutathione peroxidase activity
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + 1-chloro-2,4-dinitrobenzene
-
Saccharomyces cerevisiae S-(2,4-dinitrophenyl)glutathione + HCl
-
?

Synonyms

Synonyms Comment Organism
Ure2p mutant A122C
-
Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
Ure2p mutant A122C Saccharomyces cerevisiae
42
-
Ure2p mutant N124V Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.3
-
glutathione Ure2p mutant A122C, at 25°C and pH 8.0, in the presence of 1 mM dithiothreitol Saccharomyces cerevisiae
1.7
-
glutathione Ure2p mutant N124V, at 25°C and pH 8.0 Saccharomyces cerevisiae