KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
glutathione | at 25°C | Monopterus albus | |
0.28 | - |
1-chloro-2,4-dinitrobenzene | at 25°C | Monopterus albus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Monopterus albus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25900 | - |
2 * 25900, MALDI-TOF mass spectrometry | Monopterus albus |
26000 | - |
2 * 26000, SDS-PAGE | Monopterus albus |
52000 | - |
SDS-PAGE | Monopterus albus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Monopterus albus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
glutathione-Sepharose 6B column chromatography | Monopterus albus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Monopterus albus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13.07 | - |
after 300fold purification, at 25°C | Monopterus albus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + 1-chloro-2,4-dinitrobenzene | - |
Monopterus albus | S-(2,4-dinitrophenyl)glutathione + HCl | - |
? | |
glutathione + 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole | - |
Monopterus albus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 26000, SDS-PAGE | Monopterus albus |
homodimer | 2 * 25900, MALDI-TOF mass spectrometry | Monopterus albus |
Synonyms | Comment | Organism |
---|---|---|
GST | - |
Monopterus albus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | - |
Monopterus albus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 55 | the enzyme retains 80% of its maximum activity at 30°C and 55°C, high thermal stability with 77% of its initial activity at 45°C, retains 90% of its initial activity at 40°C, indicating GST is stable below 40°C | Monopterus albus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.44 | - |
1-chloro-2,4-dinitrobenzene | at 25°C | Monopterus albus | |
3.82 | - |
glutathione | at 25°C | Monopterus albus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | - |
Monopterus albus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | at pH 6.5 and 8.5, GST still has 62% and 72% of its maximum activity | Monopterus albus |