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Literature summary for 2.5.1.18 extracted from

  • Huang, Q.; Liang, L.; Wei, T.; Zhang, D.; Zeng, Q.
    Purification and partial characterization of glutathione transferase from the teleost Monopterus albus (2008), Comp. Biochem. Physiol. C, 147C, 96-100.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
glutathione at 25°C Monopterus albus
0.28
-
1-chloro-2,4-dinitrobenzene at 25°C Monopterus albus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Monopterus albus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25900
-
2 * 25900, MALDI-TOF mass spectrometry Monopterus albus
26000
-
2 * 26000, SDS-PAGE Monopterus albus
52000
-
SDS-PAGE Monopterus albus

Organism

Organism UniProt Comment Textmining
Monopterus albus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione-Sepharose 6B column chromatography Monopterus albus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Monopterus albus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13.07
-
after 300fold purification, at 25°C Monopterus albus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutathione + 1-chloro-2,4-dinitrobenzene
-
Monopterus albus S-(2,4-dinitrophenyl)glutathione + HCl
-
?
glutathione + 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole
-
Monopterus albus ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 26000, SDS-PAGE Monopterus albus
homodimer 2 * 25900, MALDI-TOF mass spectrometry Monopterus albus

Synonyms

Synonyms Comment Organism
GST
-
Monopterus albus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50
-
Monopterus albus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 55 the enzyme retains 80% of its maximum activity at 30°C and 55°C, high thermal stability with 77% of its initial activity at 45°C, retains 90% of its initial activity at 40°C, indicating GST is stable below 40°C Monopterus albus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.44
-
1-chloro-2,4-dinitrobenzene at 25°C Monopterus albus
3.82
-
glutathione at 25°C Monopterus albus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
Monopterus albus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 8.5 at pH 6.5 and 8.5, GST still has 62% and 72% of its maximum activity Monopterus albus